Effects of neuropeptides and mechanical loading on bone cell resorption in vitro

Yeong Min Yoo, Ji Hyun Kwag, Kyung Hwan Kim, Chi Hyun Kim

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Neuropeptides such as vasoactive intestinal peptide (VIP) and calcitonin gene-related peptide (CGRP) are present in nerve fibers of bone tissues and have been suggested to potentially regulate bone remodeling. Oscillatory fluid flow (OFF)-induced shear stress is a potent signal in mechanotransduction that is capable of regulating both anabolic and catabolic bone remodeling. However, the interaction between neuropeptides and mechanical induction in bone remodeling is poorly understood. In this study, we attempted to quantify the effects of combined neuropeptides and mechanical stimuli on mRNA and protein expression related to bone resorption. Neuropeptides (VIP or CGRP) and/or OFF-induced shear stress were applied to MC3T3-E1 pre-osteoblastic cells and changes in receptor activator of nuclear factor kappa B (NF-κB) ligand (RANKL) and osteoprotegerin (OPG) mRNA and protein levels were quantified. Neuropeptides and OFF-induced shear stress similarly decreased RANKL and increased OPG levels compared to control. Changes were not further enhanced with combined neuropeptides and OFF-induced shear stress. These results suggest that neuropeptides CGRP and VIP have an important role in suppressing bone resorptive activities through RANKL/OPG pathway, similar to mechanical loading.

Original languageEnglish
Pages (from-to)5874-5883
Number of pages10
JournalInternational journal of molecular sciences
Volume15
Issue number4
DOIs
Publication statusPublished - 2014 Apr 8

Fingerprint

Bone Resorption
Neuropeptides
bones
peptides
Bone
shear stress
fluid flow
Osteoprotegerin
cells
Calcitonin Gene-Related Peptide
Shear stress
Flow of fluids
Bone Remodeling
Vasoactive Intestinal Peptide
genes
proteins
nerve fibers
RANK Ligand
Proteins
Bone and Bones

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Yoo, Yeong Min ; Kwag, Ji Hyun ; Kim, Kyung Hwan ; Kim, Chi Hyun. / Effects of neuropeptides and mechanical loading on bone cell resorption in vitro. In: International journal of molecular sciences. 2014 ; Vol. 15, No. 4. pp. 5874-5883.
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Effects of neuropeptides and mechanical loading on bone cell resorption in vitro. / Yoo, Yeong Min; Kwag, Ji Hyun; Kim, Kyung Hwan; Kim, Chi Hyun.

In: International journal of molecular sciences, Vol. 15, No. 4, 08.04.2014, p. 5874-5883.

Research output: Contribution to journalArticle

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