Effects of neutralization by soluble ABH antigens produced by transplanted kidneys from ABO-incompatible secretor donors

Jieun Kim, Sinyoung Kim, In Sik Hwang, Jong Rak Choi, Jae Geun Lee, Yu Seun Kim, Myoung Soo Kim, Hyun Ok Kim

Research output: Contribution to journalArticle

Abstract

Background: Grafts survive despite blood group antigens on the transplant being continuously exposed to antibodies in the blood of recipients in ABO-incompatible kidney transplantation (ABOi KT), owing to the mechanism of accommodation. We analyzed the immunodynamics of soluble ABH antigens in allografts from secretor donors and the influence of such immunodynamics on accommodation and subsequent graft survival in ABOi KT. Methods: The genotype of a known human β-galactoside α-1,2-fucosyltransferase gene (FUT2), which determines soluble ABH antigen secretor status, was established in 32 donors for ABOi KT at the Severance Hospital, from June 2010 to July 2015. Clinical outcomes of recipients, such as anti-A/B antibody titer change, renal function, and graft survival, were evaluated. Results: Twenty-five donors were secretors (78.1%), and seven were nonsecretors (21.9%). The frequency of anti-A/B IgG or IgM antibody titer elevation or reduction post-transplantation was not significantly related to donor secretor status. However, IgM titer was rapidly reduced in recipients transplanted from nonsecretor donors (P=0.01), which could be explained by the lack of absorption effect of soluble antigens, enhancing the binding of antibodies to antigens in the allografts. Interestingly, soluble ABH antigens did not affect rejection-free graft survival, which may be due to the nature of β-galactoside α-1,2-fucosyltransferase. Conclusions: Soluble ABH antigens produced by transplanted kidneys from secretor donors played a role in inducing accommodation within three months of KT through neutralization; however, major graft outcomes were not affected.

Original languageEnglish
Pages (from-to)254-260
Number of pages7
JournalAnnals of laboratory medicine
Volume37
Issue number3
DOIs
Publication statusPublished - 2017 Jan 1

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Grafts
Kidney
Antigens
Graft Survival
Kidney Transplantation
Galactosides
Antibodies
Transplants
Allografts
Immunoglobulin M
Blocking Antibodies
Blood Group Antigens
Blood
Immunoglobulin G
Genes
Transplantation
Genotype
galactoside 2-alpha-L-fucosyltransferase

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Kim, Jieun ; Kim, Sinyoung ; Hwang, In Sik ; Choi, Jong Rak ; Lee, Jae Geun ; Kim, Yu Seun ; Kim, Myoung Soo ; Kim, Hyun Ok. / Effects of neutralization by soluble ABH antigens produced by transplanted kidneys from ABO-incompatible secretor donors. In: Annals of laboratory medicine. 2017 ; Vol. 37, No. 3. pp. 254-260.
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abstract = "Background: Grafts survive despite blood group antigens on the transplant being continuously exposed to antibodies in the blood of recipients in ABO-incompatible kidney transplantation (ABOi KT), owing to the mechanism of accommodation. We analyzed the immunodynamics of soluble ABH antigens in allografts from secretor donors and the influence of such immunodynamics on accommodation and subsequent graft survival in ABOi KT. Methods: The genotype of a known human β-galactoside α-1,2-fucosyltransferase gene (FUT2), which determines soluble ABH antigen secretor status, was established in 32 donors for ABOi KT at the Severance Hospital, from June 2010 to July 2015. Clinical outcomes of recipients, such as anti-A/B antibody titer change, renal function, and graft survival, were evaluated. Results: Twenty-five donors were secretors (78.1{\%}), and seven were nonsecretors (21.9{\%}). The frequency of anti-A/B IgG or IgM antibody titer elevation or reduction post-transplantation was not significantly related to donor secretor status. However, IgM titer was rapidly reduced in recipients transplanted from nonsecretor donors (P=0.01), which could be explained by the lack of absorption effect of soluble antigens, enhancing the binding of antibodies to antigens in the allografts. Interestingly, soluble ABH antigens did not affect rejection-free graft survival, which may be due to the nature of β-galactoside α-1,2-fucosyltransferase. Conclusions: Soluble ABH antigens produced by transplanted kidneys from secretor donors played a role in inducing accommodation within three months of KT through neutralization; however, major graft outcomes were not affected.",
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Effects of neutralization by soluble ABH antigens produced by transplanted kidneys from ABO-incompatible secretor donors. / Kim, Jieun; Kim, Sinyoung; Hwang, In Sik; Choi, Jong Rak; Lee, Jae Geun; Kim, Yu Seun; Kim, Myoung Soo; Kim, Hyun Ok.

In: Annals of laboratory medicine, Vol. 37, No. 3, 01.01.2017, p. 254-260.

Research output: Contribution to journalArticle

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T1 - Effects of neutralization by soluble ABH antigens produced by transplanted kidneys from ABO-incompatible secretor donors

AU - Kim, Jieun

AU - Kim, Sinyoung

AU - Hwang, In Sik

AU - Choi, Jong Rak

AU - Lee, Jae Geun

AU - Kim, Yu Seun

AU - Kim, Myoung Soo

AU - Kim, Hyun Ok

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N2 - Background: Grafts survive despite blood group antigens on the transplant being continuously exposed to antibodies in the blood of recipients in ABO-incompatible kidney transplantation (ABOi KT), owing to the mechanism of accommodation. We analyzed the immunodynamics of soluble ABH antigens in allografts from secretor donors and the influence of such immunodynamics on accommodation and subsequent graft survival in ABOi KT. Methods: The genotype of a known human β-galactoside α-1,2-fucosyltransferase gene (FUT2), which determines soluble ABH antigen secretor status, was established in 32 donors for ABOi KT at the Severance Hospital, from June 2010 to July 2015. Clinical outcomes of recipients, such as anti-A/B antibody titer change, renal function, and graft survival, were evaluated. Results: Twenty-five donors were secretors (78.1%), and seven were nonsecretors (21.9%). The frequency of anti-A/B IgG or IgM antibody titer elevation or reduction post-transplantation was not significantly related to donor secretor status. However, IgM titer was rapidly reduced in recipients transplanted from nonsecretor donors (P=0.01), which could be explained by the lack of absorption effect of soluble antigens, enhancing the binding of antibodies to antigens in the allografts. Interestingly, soluble ABH antigens did not affect rejection-free graft survival, which may be due to the nature of β-galactoside α-1,2-fucosyltransferase. Conclusions: Soluble ABH antigens produced by transplanted kidneys from secretor donors played a role in inducing accommodation within three months of KT through neutralization; however, major graft outcomes were not affected.

AB - Background: Grafts survive despite blood group antigens on the transplant being continuously exposed to antibodies in the blood of recipients in ABO-incompatible kidney transplantation (ABOi KT), owing to the mechanism of accommodation. We analyzed the immunodynamics of soluble ABH antigens in allografts from secretor donors and the influence of such immunodynamics on accommodation and subsequent graft survival in ABOi KT. Methods: The genotype of a known human β-galactoside α-1,2-fucosyltransferase gene (FUT2), which determines soluble ABH antigen secretor status, was established in 32 donors for ABOi KT at the Severance Hospital, from June 2010 to July 2015. Clinical outcomes of recipients, such as anti-A/B antibody titer change, renal function, and graft survival, were evaluated. Results: Twenty-five donors were secretors (78.1%), and seven were nonsecretors (21.9%). The frequency of anti-A/B IgG or IgM antibody titer elevation or reduction post-transplantation was not significantly related to donor secretor status. However, IgM titer was rapidly reduced in recipients transplanted from nonsecretor donors (P=0.01), which could be explained by the lack of absorption effect of soluble antigens, enhancing the binding of antibodies to antigens in the allografts. Interestingly, soluble ABH antigens did not affect rejection-free graft survival, which may be due to the nature of β-galactoside α-1,2-fucosyltransferase. Conclusions: Soluble ABH antigens produced by transplanted kidneys from secretor donors played a role in inducing accommodation within three months of KT through neutralization; however, major graft outcomes were not affected.

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