Effects of nonionic surfactant on enzymatic hydrolysis of used newspaper

Jin Won Park, Yasuyuki Takahata, Toshio Kajiuchi, Takashi Akehata

Research output: Contribution to journalLetter

120 Citations (Scopus)

Abstract

Effects of five types of nonionic surfactant having a polyoxyethylene glycol (POG) group on enzymatic hydrolysis of used newspaper were studied. The surfactants examined in this work always enhanced the saccharification. The optimum surfactant concentration was 0.05% (wt/substrate wt) in the case of POG(21) sorbitane oleic ester. Among the surfactants, POG phenyl ether types showed the highest enhancement effect, for example, with two times higher conversion at 80 h than that without surfactant. Using POG nonylphenyl ether series, the effects of surfactant were considered from the point of the HLB (hydrophile–lypophile balance) value. The GFC (gel filtration chromatography) analysis of free enzyme quantity were also done to study the effect of surfactant on enzyme adsorption onto substrate. As the HLB value increased, the free enzyme quantity and the conversion both increased. It appears that surfactants help the enzyme to desorb from the binding site on the substrate surface after the completion of saccharification at that site.

Original languageEnglish
Pages (from-to)117-120
Number of pages4
JournalBiotechnology and Bioengineering
Volume39
Issue number1
DOIs
Publication statusPublished - 1992 Jan 5

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Newspapers
Enzymatic hydrolysis
Nonionic surfactants
Surface-Active Agents
Hydrolysis
Surface active agents
Glycols
Polyethylene glycols
Enzymes
Saccharification
Ethers
Substrates
Binding sites
Chromatography
Ether
Esters
Adsorption
Gels
Gel Chromatography
Binding Sites

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Park, Jin Won ; Takahata, Yasuyuki ; Kajiuchi, Toshio ; Akehata, Takashi. / Effects of nonionic surfactant on enzymatic hydrolysis of used newspaper. In: Biotechnology and Bioengineering. 1992 ; Vol. 39, No. 1. pp. 117-120.
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Effects of nonionic surfactant on enzymatic hydrolysis of used newspaper. / Park, Jin Won; Takahata, Yasuyuki; Kajiuchi, Toshio; Akehata, Takashi.

In: Biotechnology and Bioengineering, Vol. 39, No. 1, 05.01.1992, p. 117-120.

Research output: Contribution to journalLetter

TY - JOUR

T1 - Effects of nonionic surfactant on enzymatic hydrolysis of used newspaper

AU - Park, Jin Won

AU - Takahata, Yasuyuki

AU - Kajiuchi, Toshio

AU - Akehata, Takashi

PY - 1992/1/5

Y1 - 1992/1/5

N2 - Effects of five types of nonionic surfactant having a polyoxyethylene glycol (POG) group on enzymatic hydrolysis of used newspaper were studied. The surfactants examined in this work always enhanced the saccharification. The optimum surfactant concentration was 0.05% (wt/substrate wt) in the case of POG(21) sorbitane oleic ester. Among the surfactants, POG phenyl ether types showed the highest enhancement effect, for example, with two times higher conversion at 80 h than that without surfactant. Using POG nonylphenyl ether series, the effects of surfactant were considered from the point of the HLB (hydrophile–lypophile balance) value. The GFC (gel filtration chromatography) analysis of free enzyme quantity were also done to study the effect of surfactant on enzyme adsorption onto substrate. As the HLB value increased, the free enzyme quantity and the conversion both increased. It appears that surfactants help the enzyme to desorb from the binding site on the substrate surface after the completion of saccharification at that site.

AB - Effects of five types of nonionic surfactant having a polyoxyethylene glycol (POG) group on enzymatic hydrolysis of used newspaper were studied. The surfactants examined in this work always enhanced the saccharification. The optimum surfactant concentration was 0.05% (wt/substrate wt) in the case of POG(21) sorbitane oleic ester. Among the surfactants, POG phenyl ether types showed the highest enhancement effect, for example, with two times higher conversion at 80 h than that without surfactant. Using POG nonylphenyl ether series, the effects of surfactant were considered from the point of the HLB (hydrophile–lypophile balance) value. The GFC (gel filtration chromatography) analysis of free enzyme quantity were also done to study the effect of surfactant on enzyme adsorption onto substrate. As the HLB value increased, the free enzyme quantity and the conversion both increased. It appears that surfactants help the enzyme to desorb from the binding site on the substrate surface after the completion of saccharification at that site.

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