Effects of omega-3 fatty acids on apoptosis of human gastric epithelial cells exposed to silica-immobilized glucose oxidase

Ji Hoon Yu, Sin Gun Kang, Un Young Jung, Chul-Ho Jun, Hye Young Kim

Research output: Chapter in Book/Report/Conference proceedingConference contribution

16 Citations (Scopus)

Abstract

Oxidative stress plays a critical role in apoptosis of gastric epithelial cells. Omega-3 fatty acids show anti-inflammatory and/or anticancer effects and regulate apoptosis in various cells. In this study, we aimed to investigate whether omega-3 fatty acids inhibit oxidative stress-induced apoptosis of gastric epithelial cells. The cells received oxidative stress caused by silica-immobilized glucose oxidase acting on β-d-glucose and cultured in the absence or presence of α-linolenic acid or docosahexanoic acid. Viable cell numbers, levels of H2O2 in the medium, DNA fragmentation, and protein levels of p53 and Bax were determined. As a result, silica-immobilized glucose oxidase acting on β-d-glucose consistently and reproducibly produced H2O2, which decreased cell viability and increased DNA fragmentation of the cells. Omega-3 fatty acids inhibited oxidative stress-induced cell death, DNA fragmentation, and induction of p53 and Bax of the cells. The silica-immobilized glucose oxidase could be a useful tool for studies on oxidative stress-induced cellular events because it is reusable and forms a stable enzyme system acting on glucose. Omega-3 fatty acids may be beneficial for preventing oxidative stress-induced apoptosis by inhibiting apoptotic gene expression and DNA fragmentation of gastric epithelial cells.

Original languageEnglish
Title of host publicationNatural Compounds and Their Role in Apoptotic Cell Signaling Pathways
PublisherBlackwell Publishing Inc.
Pages359-364
Number of pages6
ISBN (Print)9781573317375
DOIs
Publication statusPublished - 2009 Jan 1

Publication series

NameAnnals of the New York Academy of Sciences
Volume1171
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Glucose Oxidase
Oxidative stress
Omega-3 Fatty Acids
Silicon Dioxide
Stomach
Oxidative Stress
Epithelial Cells
DNA Fragmentation
Apoptosis
DNA
Glucose
alpha-Linolenic Acid
Cell death
Gene expression
Silica
Cells
Fatty Acids
Cell Survival
Cell Death
Anti-Inflammatory Agents

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Yu, J. H., Kang, S. G., Jung, U. Y., Jun, C-H., & Kim, H. Y. (2009). Effects of omega-3 fatty acids on apoptosis of human gastric epithelial cells exposed to silica-immobilized glucose oxidase. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways (pp. 359-364). (Annals of the New York Academy of Sciences; Vol. 1171). Blackwell Publishing Inc.. https://doi.org/10.1111/j.1749-6632.2009.04703.x
Yu, Ji Hoon ; Kang, Sin Gun ; Jung, Un Young ; Jun, Chul-Ho ; Kim, Hye Young. / Effects of omega-3 fatty acids on apoptosis of human gastric epithelial cells exposed to silica-immobilized glucose oxidase. Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc., 2009. pp. 359-364 (Annals of the New York Academy of Sciences).
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Yu, JH, Kang, SG, Jung, UY, Jun, C-H & Kim, HY 2009, Effects of omega-3 fatty acids on apoptosis of human gastric epithelial cells exposed to silica-immobilized glucose oxidase. in Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Annals of the New York Academy of Sciences, vol. 1171, Blackwell Publishing Inc., pp. 359-364. https://doi.org/10.1111/j.1749-6632.2009.04703.x

Effects of omega-3 fatty acids on apoptosis of human gastric epithelial cells exposed to silica-immobilized glucose oxidase. / Yu, Ji Hoon; Kang, Sin Gun; Jung, Un Young; Jun, Chul-Ho; Kim, Hye Young.

Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc., 2009. p. 359-364 (Annals of the New York Academy of Sciences; Vol. 1171).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - Oxidative stress plays a critical role in apoptosis of gastric epithelial cells. Omega-3 fatty acids show anti-inflammatory and/or anticancer effects and regulate apoptosis in various cells. In this study, we aimed to investigate whether omega-3 fatty acids inhibit oxidative stress-induced apoptosis of gastric epithelial cells. The cells received oxidative stress caused by silica-immobilized glucose oxidase acting on β-d-glucose and cultured in the absence or presence of α-linolenic acid or docosahexanoic acid. Viable cell numbers, levels of H2O2 in the medium, DNA fragmentation, and protein levels of p53 and Bax were determined. As a result, silica-immobilized glucose oxidase acting on β-d-glucose consistently and reproducibly produced H2O2, which decreased cell viability and increased DNA fragmentation of the cells. Omega-3 fatty acids inhibited oxidative stress-induced cell death, DNA fragmentation, and induction of p53 and Bax of the cells. The silica-immobilized glucose oxidase could be a useful tool for studies on oxidative stress-induced cellular events because it is reusable and forms a stable enzyme system acting on glucose. Omega-3 fatty acids may be beneficial for preventing oxidative stress-induced apoptosis by inhibiting apoptotic gene expression and DNA fragmentation of gastric epithelial cells.

AB - Oxidative stress plays a critical role in apoptosis of gastric epithelial cells. Omega-3 fatty acids show anti-inflammatory and/or anticancer effects and regulate apoptosis in various cells. In this study, we aimed to investigate whether omega-3 fatty acids inhibit oxidative stress-induced apoptosis of gastric epithelial cells. The cells received oxidative stress caused by silica-immobilized glucose oxidase acting on β-d-glucose and cultured in the absence or presence of α-linolenic acid or docosahexanoic acid. Viable cell numbers, levels of H2O2 in the medium, DNA fragmentation, and protein levels of p53 and Bax were determined. As a result, silica-immobilized glucose oxidase acting on β-d-glucose consistently and reproducibly produced H2O2, which decreased cell viability and increased DNA fragmentation of the cells. Omega-3 fatty acids inhibited oxidative stress-induced cell death, DNA fragmentation, and induction of p53 and Bax of the cells. The silica-immobilized glucose oxidase could be a useful tool for studies on oxidative stress-induced cellular events because it is reusable and forms a stable enzyme system acting on glucose. Omega-3 fatty acids may be beneficial for preventing oxidative stress-induced apoptosis by inhibiting apoptotic gene expression and DNA fragmentation of gastric epithelial cells.

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Yu JH, Kang SG, Jung UY, Jun C-H, Kim HY. Effects of omega-3 fatty acids on apoptosis of human gastric epithelial cells exposed to silica-immobilized glucose oxidase. In Natural Compounds and Their Role in Apoptotic Cell Signaling Pathways. Blackwell Publishing Inc. 2009. p. 359-364. (Annals of the New York Academy of Sciences). https://doi.org/10.1111/j.1749-6632.2009.04703.x