Effects of oxytocin on cell proliferation in a corticotroph adenoma cell line

Jung Soo Lim, Young Woo Eom, Eun Soo Lee, Hyeong Ju Kwon, Ja Young Kwon, Junjeong Choi, Choon Hee Chung, Young Suk Jo, Eun Jig Lee

Research output: Contribution to journalArticle

Abstract

Background: Oxytocin (OXT) has been reported to act as a growth regulator in various tumor cells. However, there is a paucity of data on the influence of OXT on cell proliferation of corticotroph adenomas. This study aimed to examine whether OXT affects cell growth in pituitary tumor cell lines (AtT20 and GH3 cells) with a focus on corticotroph adenoma cells. Methods: Reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay were conducted with AtT20 cells to confirm the effects of OXT on hormonal activity; flow cytometry was used to assess changes in the cell cycle after OXT treatment. Moreover, the impact of OXT on proliferating cell nuclear antigen (PCNA), nuclear factor κB, and mitogen-activated protein kinase signaling pathway was analyzed by Western blot. Results: OXT treatment of 50 nM changed the gene expression of OXT receptor and pro-opiomelanocortin within a short time. In addition, OXT significantly reduced adrenocorticotropic hormone secretion within 1 hour. S and G2/M populations of AtT20 cells treated with OXT for 24 hours were significantly decreased compared to the control. Furthermore, OXT treatment decreased the protein levels of PCNA and phosphorylated extracellular-signal-regulated kinase (P-ERK) in AtT20 cells. Conclusion: Although the cytotoxic effect of OXT in AtT20 cells was not definite, OXT may blunt cell proliferation of corticotroph adenomas by altering the cell cycle or reducing PCNA and P-ERK levels. Further research is required to investigate the role of OXT as a potential therapeutic target in corticotroph adenomas.

Original languageEnglish
Pages (from-to)302-313
Number of pages12
JournalEndocrinology and Metabolism
Volume34
Issue number3
DOIs
Publication statusPublished - 2019 Jan 1

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ACTH-Secreting Pituitary Adenoma
Oxytocin
Cell Proliferation
Cell Line
Proliferating Cell Nuclear Antigen
Extracellular Signal-Regulated MAP Kinases
Cell Cycle
Oxytocin Receptors
Pro-Opiomelanocortin
Pituitary Neoplasms
Therapeutics
Growth

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Lim, Jung Soo ; Eom, Young Woo ; Lee, Eun Soo ; Kwon, Hyeong Ju ; Kwon, Ja Young ; Choi, Junjeong ; Chung, Choon Hee ; Jo, Young Suk ; Lee, Eun Jig. / Effects of oxytocin on cell proliferation in a corticotroph adenoma cell line. In: Endocrinology and Metabolism. 2019 ; Vol. 34, No. 3. pp. 302-313.
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abstract = "Background: Oxytocin (OXT) has been reported to act as a growth regulator in various tumor cells. However, there is a paucity of data on the influence of OXT on cell proliferation of corticotroph adenomas. This study aimed to examine whether OXT affects cell growth in pituitary tumor cell lines (AtT20 and GH3 cells) with a focus on corticotroph adenoma cells. Methods: Reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay were conducted with AtT20 cells to confirm the effects of OXT on hormonal activity; flow cytometry was used to assess changes in the cell cycle after OXT treatment. Moreover, the impact of OXT on proliferating cell nuclear antigen (PCNA), nuclear factor κB, and mitogen-activated protein kinase signaling pathway was analyzed by Western blot. Results: OXT treatment of 50 nM changed the gene expression of OXT receptor and pro-opiomelanocortin within a short time. In addition, OXT significantly reduced adrenocorticotropic hormone secretion within 1 hour. S and G2/M populations of AtT20 cells treated with OXT for 24 hours were significantly decreased compared to the control. Furthermore, OXT treatment decreased the protein levels of PCNA and phosphorylated extracellular-signal-regulated kinase (P-ERK) in AtT20 cells. Conclusion: Although the cytotoxic effect of OXT in AtT20 cells was not definite, OXT may blunt cell proliferation of corticotroph adenomas by altering the cell cycle or reducing PCNA and P-ERK levels. Further research is required to investigate the role of OXT as a potential therapeutic target in corticotroph adenomas.",
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Effects of oxytocin on cell proliferation in a corticotroph adenoma cell line. / Lim, Jung Soo; Eom, Young Woo; Lee, Eun Soo; Kwon, Hyeong Ju; Kwon, Ja Young; Choi, Junjeong; Chung, Choon Hee; Jo, Young Suk; Lee, Eun Jig.

In: Endocrinology and Metabolism, Vol. 34, No. 3, 01.01.2019, p. 302-313.

Research output: Contribution to journalArticle

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AU - Lim, Jung Soo

AU - Eom, Young Woo

AU - Lee, Eun Soo

AU - Kwon, Hyeong Ju

AU - Kwon, Ja Young

AU - Choi, Junjeong

AU - Chung, Choon Hee

AU - Jo, Young Suk

AU - Lee, Eun Jig

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N2 - Background: Oxytocin (OXT) has been reported to act as a growth regulator in various tumor cells. However, there is a paucity of data on the influence of OXT on cell proliferation of corticotroph adenomas. This study aimed to examine whether OXT affects cell growth in pituitary tumor cell lines (AtT20 and GH3 cells) with a focus on corticotroph adenoma cells. Methods: Reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay were conducted with AtT20 cells to confirm the effects of OXT on hormonal activity; flow cytometry was used to assess changes in the cell cycle after OXT treatment. Moreover, the impact of OXT on proliferating cell nuclear antigen (PCNA), nuclear factor κB, and mitogen-activated protein kinase signaling pathway was analyzed by Western blot. Results: OXT treatment of 50 nM changed the gene expression of OXT receptor and pro-opiomelanocortin within a short time. In addition, OXT significantly reduced adrenocorticotropic hormone secretion within 1 hour. S and G2/M populations of AtT20 cells treated with OXT for 24 hours were significantly decreased compared to the control. Furthermore, OXT treatment decreased the protein levels of PCNA and phosphorylated extracellular-signal-regulated kinase (P-ERK) in AtT20 cells. Conclusion: Although the cytotoxic effect of OXT in AtT20 cells was not definite, OXT may blunt cell proliferation of corticotroph adenomas by altering the cell cycle or reducing PCNA and P-ERK levels. Further research is required to investigate the role of OXT as a potential therapeutic target in corticotroph adenomas.

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