Effects of ozone exposure on the ocular surface

Hun Lee, Eungkweon Kim, Sang Won Kang, Jae Hoon Kim, Hye Jeong Hwang, Tae-im Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Changes in the ocular surface induced by ozone have received limited research attention. Here, we investigate the effects of ozone exposure on the integrity of the ocular surface, the production of inflammatory cytokines in tears, and changes in mucin-secreting cells in a mouse model. In addition, ozone-induced nuclear factor-κB (NF-κB)-mediated inflammatory processes were evaluated in cultured human conjunctival epithelial cells. In vivo, ozone induced the breakdown of corneal epithelial integrity, decreased the number of mucin-secreting cells, and induced the production of inflammatory cytokines, without altering tear volume. In vitro, ozone exposure led to increases in NF-κB nuclear translocation, κB-dependent transcriptional activity, NF-κB inhibitor a (IκBα) proteolysis, and expression of phosphorylated IκBα (p-IκBα), but did not cause cytotoxicity or cellular apoptosis. In addition, ozone induced the expression of inflammatory cytokines, Toll-like receptors, and C-C chemokine receptors, but decreased the expression of mucins. Furthermore, inhibition of NF-κB with pyrrolidine dithiocarbamate before exposure of cultured human conjunctival epithelial cells to ozone prevented changes in IκBα and p-IκBα levels in association with a decrease in the levels of inflammatory cytokines. Therefore, we conclude that ozone exposure interferes with ocular surface integrity and induces inflammation involving NF-κB-mediated processes at the level (and/or upstream) of IκBα. Understanding the role of ozone in the initiation of inflammatory processes on the animal ocular surface and in cultured human conjunctival epithelial cells can help elucidate the pathogenesis of ocular surface damage and suggest protective strategies for preserving a healthy ocular surface against ozone exposure.

Original languageEnglish
Pages (from-to)78-89
Number of pages12
JournalFree Radical Biology and Medicine
Volume63
DOIs
Publication statusPublished - 2013 Jan 1

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Ozone
Mucins
Cytokines
Epithelial Cells
Tears
Proteolysis
CC Chemokines
Chemokine Receptors
Toll-Like Receptors
Cytotoxicity
Animals
Association reactions
Apoptosis
Inflammation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology (medical)

Cite this

Lee, Hun ; Kim, Eungkweon ; Kang, Sang Won ; Kim, Jae Hoon ; Hwang, Hye Jeong ; Kim, Tae-im. / Effects of ozone exposure on the ocular surface. In: Free Radical Biology and Medicine. 2013 ; Vol. 63. pp. 78-89.
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Effects of ozone exposure on the ocular surface. / Lee, Hun; Kim, Eungkweon; Kang, Sang Won; Kim, Jae Hoon; Hwang, Hye Jeong; Kim, Tae-im.

In: Free Radical Biology and Medicine, Vol. 63, 01.01.2013, p. 78-89.

Research output: Contribution to journalArticle

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