Effects of PDMS curing ratio and 3D micro-pyramid structure on the formation of an in vitro neural network

Ahmi Choi, Jae Young Kim, Jongeun Lee, Hyo il Jung

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

An in vitro neural network can provide a model to investigate the signaling processes that regulate the body functions. Using polydimethylsiloxane (PDMS) multilayer micro-structures to construct a neural network, the bonding between the layers can be improved by modulation of the curing ratio. In this study, we found that as the curing ratio increased from 10:1 to 10:4, the contact angle decreased from 111.69° to 102.08° and the surface energy and roughness increased. For adhesion and proliferation, the hippocampal primary neural cells preferred a PDMS surface with a 10:1 curing ratio to other surfaces. In addition, 3D PDMS micro-pyramid array allows the primary hippocampal neuron to pattern a network without any surface treatment and the network was verified by immunocytochemistry. These results may suggest the optimum PDMS curing ratio to apply in constructing a cell device for in vitro neural network formation as well as the potential of 3D structure fabrication which allows us to construct neural network without any surface treatment.

Original languageEnglish
JournalCurrent Applied Physics
Volume9
Issue number4 SUPPL.
DOIs
Publication statusPublished - 2009 Jul 1

Fingerprint

Polydimethylsiloxane
curing
pyramids
Curing
Neural networks
Microstructure
Surface treatment
surface treatment
Interfacial energy
Neurons
Contact angle
Multilayers
Adhesion
Surface roughness
Modulation
cells
neurons
Fabrication
surface energy
baysilon

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

@article{3071db4159254fad823fb3c29b091328,
title = "Effects of PDMS curing ratio and 3D micro-pyramid structure on the formation of an in vitro neural network",
abstract = "An in vitro neural network can provide a model to investigate the signaling processes that regulate the body functions. Using polydimethylsiloxane (PDMS) multilayer micro-structures to construct a neural network, the bonding between the layers can be improved by modulation of the curing ratio. In this study, we found that as the curing ratio increased from 10:1 to 10:4, the contact angle decreased from 111.69° to 102.08° and the surface energy and roughness increased. For adhesion and proliferation, the hippocampal primary neural cells preferred a PDMS surface with a 10:1 curing ratio to other surfaces. In addition, 3D PDMS micro-pyramid array allows the primary hippocampal neuron to pattern a network without any surface treatment and the network was verified by immunocytochemistry. These results may suggest the optimum PDMS curing ratio to apply in constructing a cell device for in vitro neural network formation as well as the potential of 3D structure fabrication which allows us to construct neural network without any surface treatment.",
author = "Ahmi Choi and Kim, {Jae Young} and Jongeun Lee and Jung, {Hyo il}",
year = "2009",
month = "7",
day = "1",
doi = "10.1016/j.cap.2009.06.029",
language = "English",
volume = "9",
journal = "Current Applied Physics",
issn = "1567-1739",
publisher = "Elsevier",
number = "4 SUPPL.",

}

Effects of PDMS curing ratio and 3D micro-pyramid structure on the formation of an in vitro neural network. / Choi, Ahmi; Kim, Jae Young; Lee, Jongeun; Jung, Hyo il.

In: Current Applied Physics, Vol. 9, No. 4 SUPPL., 01.07.2009.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of PDMS curing ratio and 3D micro-pyramid structure on the formation of an in vitro neural network

AU - Choi, Ahmi

AU - Kim, Jae Young

AU - Lee, Jongeun

AU - Jung, Hyo il

PY - 2009/7/1

Y1 - 2009/7/1

N2 - An in vitro neural network can provide a model to investigate the signaling processes that regulate the body functions. Using polydimethylsiloxane (PDMS) multilayer micro-structures to construct a neural network, the bonding between the layers can be improved by modulation of the curing ratio. In this study, we found that as the curing ratio increased from 10:1 to 10:4, the contact angle decreased from 111.69° to 102.08° and the surface energy and roughness increased. For adhesion and proliferation, the hippocampal primary neural cells preferred a PDMS surface with a 10:1 curing ratio to other surfaces. In addition, 3D PDMS micro-pyramid array allows the primary hippocampal neuron to pattern a network without any surface treatment and the network was verified by immunocytochemistry. These results may suggest the optimum PDMS curing ratio to apply in constructing a cell device for in vitro neural network formation as well as the potential of 3D structure fabrication which allows us to construct neural network without any surface treatment.

AB - An in vitro neural network can provide a model to investigate the signaling processes that regulate the body functions. Using polydimethylsiloxane (PDMS) multilayer micro-structures to construct a neural network, the bonding between the layers can be improved by modulation of the curing ratio. In this study, we found that as the curing ratio increased from 10:1 to 10:4, the contact angle decreased from 111.69° to 102.08° and the surface energy and roughness increased. For adhesion and proliferation, the hippocampal primary neural cells preferred a PDMS surface with a 10:1 curing ratio to other surfaces. In addition, 3D PDMS micro-pyramid array allows the primary hippocampal neuron to pattern a network without any surface treatment and the network was verified by immunocytochemistry. These results may suggest the optimum PDMS curing ratio to apply in constructing a cell device for in vitro neural network formation as well as the potential of 3D structure fabrication which allows us to construct neural network without any surface treatment.

UR - http://www.scopus.com/inward/record.url?scp=71849110671&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71849110671&partnerID=8YFLogxK

U2 - 10.1016/j.cap.2009.06.029

DO - 10.1016/j.cap.2009.06.029

M3 - Article

VL - 9

JO - Current Applied Physics

JF - Current Applied Physics

SN - 1567-1739

IS - 4 SUPPL.

ER -