Effects of pH and dissolved oxygen on cellulose production by Acetobacter xylinum BRC5 in agitated culture

Jung Wook Hwang, Young Kook Yang, Jae-Kwan Hwang, Yu Ryang Pyun, Yu Sam Kim

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Acetobacter xylinum BRC5 was cultivated in a jar fermentor using glucose as the sole carbon source. Strain BRC5 oxidized almost all of the glucose to gluconic acid; thereafter, it biosynthesized cellulose by utilizing gluconic acid accumulated in the broth. The optimal pH for metabolizing glucose to gluconic acid was 4.0, while a pH of 5.5 was preferred for cell growth and cellulose production from the accumulated gluconic acid in the medium. Shifting the pH from 4.0 to 5.5 during the cellulose production phase in batch cultures improved cellulose production and reduced the total fermentation time, compared to batch cultures at constant pH. In constant fed-batch culture, 10 g/l of cellulose was obtained from 40 g/l of glucose, a yield which was approximately 2-fold higher than in batch culture with the same initial glucose concentration, even without control of the level of dissolved oxygen. The highest cellulose yield was obtained in fed-batch cultures in which the dissolved oxygen concentration was controlled at 10% saturation. Control of pH and dissolved oxygen to optimal levels was effective for improving the production rate and yield of cellulose, to achieve a high cellulose productivity of 0.3 g cellulose/l·h. Approximately 15 g/l of cellulose was considered to be the highest yield obtainable using conventional fermentors because the culture broth then became too viscous to allow satisfactory aeration.

Original languageEnglish
Pages (from-to)183-188
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume88
Issue number2
DOIs
Publication statusPublished - 1999 Jan 1

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Gluconacetobacter xylinus
Acetobacter
Dissolved oxygen
Cellulose
Oxygen
Batch Cell Culture Techniques
Glucose
Acids
Bioreactors
Cell growth
Fermentation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Hwang, Jung Wook ; Yang, Young Kook ; Hwang, Jae-Kwan ; Pyun, Yu Ryang ; Kim, Yu Sam. / Effects of pH and dissolved oxygen on cellulose production by Acetobacter xylinum BRC5 in agitated culture. In: Journal of Bioscience and Bioengineering. 1999 ; Vol. 88, No. 2. pp. 183-188.
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Effects of pH and dissolved oxygen on cellulose production by Acetobacter xylinum BRC5 in agitated culture. / Hwang, Jung Wook; Yang, Young Kook; Hwang, Jae-Kwan; Pyun, Yu Ryang; Kim, Yu Sam.

In: Journal of Bioscience and Bioengineering, Vol. 88, No. 2, 01.01.1999, p. 183-188.

Research output: Contribution to journalArticle

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