Effects of sterols on the development and aging of Caenorhabditis elegans.

Eun Young Lee, Pan Young Jeong, Sun Young Kim, Yhong Hee Shim, David J. Chitwood, Young-Ki Paik

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Although Caenorhabditis elegans lacks several components of the de novo sterol biosynthetic pathway, it requires sterols as essential nutrients. Supplemental cholesterol undergoes extensive enzymatic modification in C. elegans to form certain sterols of unknown function. Since sterol metabolism in C. elegans differs from that in other species, such as mammals and yeast, it is important to examine how sterols regulate worm physiology. To examine the functions of sterols in C. elegans, a sterol-feeding experiment was carried out and several critical parameters, such as brood size, growth rate, and life span, were measured. In addition, the change in lipid distribution in C. elegans can be both qualitatively and quantitatively determined by various methods, including staining and chromatographic techniques. Taken together, the effects of sterols on C. elegans are very prominent and can be easily assessed using the techniques described here.

Original languageEnglish
Pages (from-to)167-179
Number of pages13
JournalMethods in molecular biology (Clifton, N.J.)
Volume462
Publication statusPublished - 2009 Jan 1

Fingerprint

Caenorhabditis elegans
Sterols
Biosynthetic Pathways
Mammals
Yeasts
Cholesterol
Staining and Labeling
Lipids
Food
Growth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Lee, E. Y., Jeong, P. Y., Kim, S. Y., Shim, Y. H., Chitwood, D. J., & Paik, Y-K. (2009). Effects of sterols on the development and aging of Caenorhabditis elegans. Methods in molecular biology (Clifton, N.J.), 462, 167-179.
Lee, Eun Young ; Jeong, Pan Young ; Kim, Sun Young ; Shim, Yhong Hee ; Chitwood, David J. ; Paik, Young-Ki. / Effects of sterols on the development and aging of Caenorhabditis elegans. In: Methods in molecular biology (Clifton, N.J.). 2009 ; Vol. 462. pp. 167-179.
@article{feede1c5b747412e9c1ac4c3ef098c3a,
title = "Effects of sterols on the development and aging of Caenorhabditis elegans.",
abstract = "Although Caenorhabditis elegans lacks several components of the de novo sterol biosynthetic pathway, it requires sterols as essential nutrients. Supplemental cholesterol undergoes extensive enzymatic modification in C. elegans to form certain sterols of unknown function. Since sterol metabolism in C. elegans differs from that in other species, such as mammals and yeast, it is important to examine how sterols regulate worm physiology. To examine the functions of sterols in C. elegans, a sterol-feeding experiment was carried out and several critical parameters, such as brood size, growth rate, and life span, were measured. In addition, the change in lipid distribution in C. elegans can be both qualitatively and quantitatively determined by various methods, including staining and chromatographic techniques. Taken together, the effects of sterols on C. elegans are very prominent and can be easily assessed using the techniques described here.",
author = "Lee, {Eun Young} and Jeong, {Pan Young} and Kim, {Sun Young} and Shim, {Yhong Hee} and Chitwood, {David J.} and Young-Ki Paik",
year = "2009",
month = "1",
day = "1",
language = "English",
volume = "462",
pages = "167--179",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

Effects of sterols on the development and aging of Caenorhabditis elegans. / Lee, Eun Young; Jeong, Pan Young; Kim, Sun Young; Shim, Yhong Hee; Chitwood, David J.; Paik, Young-Ki.

In: Methods in molecular biology (Clifton, N.J.), Vol. 462, 01.01.2009, p. 167-179.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effects of sterols on the development and aging of Caenorhabditis elegans.

AU - Lee, Eun Young

AU - Jeong, Pan Young

AU - Kim, Sun Young

AU - Shim, Yhong Hee

AU - Chitwood, David J.

AU - Paik, Young-Ki

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Although Caenorhabditis elegans lacks several components of the de novo sterol biosynthetic pathway, it requires sterols as essential nutrients. Supplemental cholesterol undergoes extensive enzymatic modification in C. elegans to form certain sterols of unknown function. Since sterol metabolism in C. elegans differs from that in other species, such as mammals and yeast, it is important to examine how sterols regulate worm physiology. To examine the functions of sterols in C. elegans, a sterol-feeding experiment was carried out and several critical parameters, such as brood size, growth rate, and life span, were measured. In addition, the change in lipid distribution in C. elegans can be both qualitatively and quantitatively determined by various methods, including staining and chromatographic techniques. Taken together, the effects of sterols on C. elegans are very prominent and can be easily assessed using the techniques described here.

AB - Although Caenorhabditis elegans lacks several components of the de novo sterol biosynthetic pathway, it requires sterols as essential nutrients. Supplemental cholesterol undergoes extensive enzymatic modification in C. elegans to form certain sterols of unknown function. Since sterol metabolism in C. elegans differs from that in other species, such as mammals and yeast, it is important to examine how sterols regulate worm physiology. To examine the functions of sterols in C. elegans, a sterol-feeding experiment was carried out and several critical parameters, such as brood size, growth rate, and life span, were measured. In addition, the change in lipid distribution in C. elegans can be both qualitatively and quantitatively determined by various methods, including staining and chromatographic techniques. Taken together, the effects of sterols on C. elegans are very prominent and can be easily assessed using the techniques described here.

UR - http://www.scopus.com/inward/record.url?scp=59849092840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=59849092840&partnerID=8YFLogxK

M3 - Article

VL - 462

SP - 167

EP - 179

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -