Effects of tautomycetin on proliferation and fibronectin secretion in vascular smooth muscle cells and glomerular mesangial cells

J. H. Kim, T. Y. Lee, J. Park, H. Ha, S. W. Kang, Y. S. Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Tautomycetin (TMC), a newly developed immunosuppressive agent, induces T-lymphocyte apoptosis through the inhibition of tyrosine kinase and protein phosphatase 1. We examined the effects of TMC on platelet-derived growth factor (PDGF)-induced proliferation and extracellular matrix synthesis in cultured vascular smooth muscle cells (VSMCs) and mesangial cells (MCs) of Sprague-Dawley rats, and investigated the molecular mechanisms involved. Different concentrations of TMC were administered 1 hour before the addition of 10 ng/mL PDGF into the growth-arrested and synchronized cells. Cell proliferation was assessed by methylthiazoletetrazolium (MTT) assay, fibronectin secretion, and the activation of Akt, ERK, and p38 MAPK by Western blot analysis. PDGF increased cell proliferation, fibronectin secretion, and the activation of Akt, ERK, and p38 MAPK in both VSMCs and MCs. In both cultured cells, TMC at >1 μg/mL significantly reduced basal MTT. TMC at 100 ng/mL significantly decreased the PDGF-induced VSMC and MC proliferation. However, fibronectin secretion and the activation of Akt, ERK, and p38 MAPK were not affected by this nontoxic concentration of TMC. The present data demonstrate that low-dose TMC reduced PDGF-induced VSMC and MC proliferation without affecting the fibronectin secretion and cellular kinase activation.

Original languageEnglish
Pages (from-to)1959-1961
Number of pages3
JournalTransplantation Proceedings
Volume37
Issue number4
DOIs
Publication statusPublished - 2005 May 1

Fingerprint

Mesangial Cells
Vascular Smooth Muscle
Fibronectins
Smooth Muscle Myocytes
Platelet-Derived Growth Factor
p38 Mitogen-Activated Protein Kinases
Cell Proliferation
Protein Phosphatase 1
Immunosuppressive Agents
tautomycetin
Protein-Tyrosine Kinases
Extracellular Matrix
Sprague Dawley Rats
Cultured Cells
Phosphotransferases
Western Blotting
Apoptosis
T-Lymphocytes
Growth

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

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abstract = "Tautomycetin (TMC), a newly developed immunosuppressive agent, induces T-lymphocyte apoptosis through the inhibition of tyrosine kinase and protein phosphatase 1. We examined the effects of TMC on platelet-derived growth factor (PDGF)-induced proliferation and extracellular matrix synthesis in cultured vascular smooth muscle cells (VSMCs) and mesangial cells (MCs) of Sprague-Dawley rats, and investigated the molecular mechanisms involved. Different concentrations of TMC were administered 1 hour before the addition of 10 ng/mL PDGF into the growth-arrested and synchronized cells. Cell proliferation was assessed by methylthiazoletetrazolium (MTT) assay, fibronectin secretion, and the activation of Akt, ERK, and p38 MAPK by Western blot analysis. PDGF increased cell proliferation, fibronectin secretion, and the activation of Akt, ERK, and p38 MAPK in both VSMCs and MCs. In both cultured cells, TMC at >1 μg/mL significantly reduced basal MTT. TMC at 100 ng/mL significantly decreased the PDGF-induced VSMC and MC proliferation. However, fibronectin secretion and the activation of Akt, ERK, and p38 MAPK were not affected by this nontoxic concentration of TMC. The present data demonstrate that low-dose TMC reduced PDGF-induced VSMC and MC proliferation without affecting the fibronectin secretion and cellular kinase activation.",
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Effects of tautomycetin on proliferation and fibronectin secretion in vascular smooth muscle cells and glomerular mesangial cells. / Kim, J. H.; Lee, T. Y.; Park, J.; Ha, H.; Kang, S. W.; Kim, Y. S.

In: Transplantation Proceedings, Vol. 37, No. 4, 01.05.2005, p. 1959-1961.

Research output: Contribution to journalArticle

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