Effects of the ammonium loading rate on nitrite-oxidizing activity during nitrification at a high dose of inorganic carbon

Minkyu Choi, Kyungjin Cho, Dawoon Jeong, Yun Chul Chung, Joonhong Park, Seockheon Lee, Hyokwan Bae

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3 Citations (Scopus)


In this study, the effects of the ammonium loading rate (ALR) and inorganic carbon loading rate (ILR) on the nitrification performance and composition of a nitrifying bacterial community were investigated in a moving bed biofilm reactor, using poly(vinyl alcohol) (PVA) sponge cubes as a supporting carrier. Between the two ALRs of 0.36 and 2.16 kg-N m−1 d−1, stable partial nitritation was achieved at the higher ALR. Inorganic carbon was dosed at high levels: 33.1, 22.0, 16.4, 11.0, and 5.4 times the theoretical amount. Nonetheless, nitrification efficiency was not affected by the ILR at the two ALRs. Quantitative PCR analysis of ammonia- and nitrite-oxidizing bacteria revealed that ALR is an important determinant of partial nitritation by accumulating ammonia-oxidizing bacteria in the nitrification system. In comparison, two nitrite-oxidizing bacterial genera (Nitrobacter and Nitrospira) showed almost the same relative abundance at various ALRs and ILRs. Terminal restriction fragment length polymorphism targeting the gene of ammonia monooxygenase subunit A revealed that Nitrosomonas europaea dominated under all conditions.

Original languageEnglish
Pages (from-to)708-717
Number of pages10
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Issue number8
Publication statusPublished - 2018 Jul 3

Bibliographical note

Funding Information:
This work was supported by 2E27102 of Korea Institute of Science and Technology and Pusan National University Research Grant, 2017. This project is also supported by the “R&D Center for reduction of Non-CO2 Greenhouse gases (2017002420003)” funded by Korea Ministry of Environment (MOE) as “Global Top Environment R&D Program”.

Publisher Copyright:
© 2018, © 2018 Taylor & Francis Group, LLC.

All Science Journal Classification (ASJC) codes

  • Environmental Engineering


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