Effects of the ammonium loading rate on nitrite-oxidizing activity during nitrification at a high dose of inorganic carbon

Minkyu Choi, Kyungjin Cho, Dawoon Jeong, Yun Chul Chung, Joonhong Park, Seockheon Lee, Hyokwan Bae

Research output: Contribution to journalArticle

Abstract

In this study, the effects of the ammonium loading rate (ALR) and inorganic carbon loading rate (ILR) on the nitrification performance and composition of a nitrifying bacterial community were investigated in a moving bed biofilm reactor, using poly(vinyl alcohol) (PVA) sponge cubes as a supporting carrier. Between the two ALRs of 0.36 and 2.16 kg-N m−1 d−1, stable partial nitritation was achieved at the higher ALR. Inorganic carbon was dosed at high levels: 33.1, 22.0, 16.4, 11.0, and 5.4 times the theoretical amount. Nonetheless, nitrification efficiency was not affected by the ILR at the two ALRs. Quantitative PCR analysis of ammonia- and nitrite-oxidizing bacteria revealed that ALR is an important determinant of partial nitritation by accumulating ammonia-oxidizing bacteria in the nitrification system. In comparison, two nitrite-oxidizing bacterial genera (Nitrobacter and Nitrospira) showed almost the same relative abundance at various ALRs and ILRs. Terminal restriction fragment length polymorphism targeting the gene of ammonia monooxygenase subunit A revealed that Nitrosomonas europaea dominated under all conditions.

Original languageEnglish
Pages (from-to)708-717
Number of pages10
JournalJournal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering
Volume53
Issue number8
DOIs
Publication statusPublished - 2018 Jul 3

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Nitrification
Ammonia
Carbon
Bacteria
Biofilms
Polymorphism
Chemical analysis
Alcohols
Genes

All Science Journal Classification (ASJC) codes

  • Environmental Engineering

Cite this

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title = "Effects of the ammonium loading rate on nitrite-oxidizing activity during nitrification at a high dose of inorganic carbon",
abstract = "In this study, the effects of the ammonium loading rate (ALR) and inorganic carbon loading rate (ILR) on the nitrification performance and composition of a nitrifying bacterial community were investigated in a moving bed biofilm reactor, using poly(vinyl alcohol) (PVA) sponge cubes as a supporting carrier. Between the two ALRs of 0.36 and 2.16 kg-N m−1 d−1, stable partial nitritation was achieved at the higher ALR. Inorganic carbon was dosed at high levels: 33.1, 22.0, 16.4, 11.0, and 5.4 times the theoretical amount. Nonetheless, nitrification efficiency was not affected by the ILR at the two ALRs. Quantitative PCR analysis of ammonia- and nitrite-oxidizing bacteria revealed that ALR is an important determinant of partial nitritation by accumulating ammonia-oxidizing bacteria in the nitrification system. In comparison, two nitrite-oxidizing bacterial genera (Nitrobacter and Nitrospira) showed almost the same relative abundance at various ALRs and ILRs. Terminal restriction fragment length polymorphism targeting the gene of ammonia monooxygenase subunit A revealed that Nitrosomonas europaea dominated under all conditions.",
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Effects of the ammonium loading rate on nitrite-oxidizing activity during nitrification at a high dose of inorganic carbon. / Choi, Minkyu; Cho, Kyungjin; Jeong, Dawoon; Chung, Yun Chul; Park, Joonhong; Lee, Seockheon; Bae, Hyokwan.

In: Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental Engineering, Vol. 53, No. 8, 03.07.2018, p. 708-717.

Research output: Contribution to journalArticle

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