Effects of three calcium silicate cements on inflammatory response and mineralization-inducing potentials in a dog pulpotomy model

Chung Min Kang, Jiwon Hwang, Je Seon Song, Jae Ho Lee, Hyung Jun Choi, Yooseok Shin

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Abstract

This beagle pulpotomy study compared the inflammatory response and mineralizationinducing potential of three calcium silicate cements: ProRoot mineral trioxide aggregate (MTA) (Dentsply, Tulsa, OK, USA), OrthoMTA (BioMTA, Seoul, Korea), and Endocem MTA (Maruchi, Wonju, Korea). Exposed pulp tissues were capped with ProRoot MTA, OrthoMTA, or Endocem MTA. After 8 weeks, we extracted the teeth, then performed hematoxylin-eosin and immunohistochemical staining with osteocalcin and dentin sialoprotein. Histological evaluation comprised a scoring system with eight broad categories and analysis of calcific barrier areas. We evaluated 44 teeth capped with ProRoot MTA (n = 15), OrthoMTA (n = 18), or Endocem MTA (n = 11). Most ProRoot MTA specimens formed continuous calcific barriers; these pulps contained inflammation-free palisading patterns in the odontoblastic layer. Areas of the newly formed calcific barrier were greater with ProRoot MTA than with Endocem MTA (p = 0.006). Although dentin sialoprotein was highly expressed in all three groups, the osteocalcin expression was reduced in the OrthoMTA and Endocem MTA groups. ProRoot MTA was superior to OrthoMTA and Endocem MTA in all histological analyses. ProRoot MTA and OrthoMTA resulted in reduced pulpal inflammation and more complete calcific barrier formation, whereas Endocem MTA caused a lower level of calcific barrier continuity with tunnel defects.

Original languageEnglish
Article number899
JournalMaterials
Volume11
Issue number6
DOIs
Publication statusPublished - 2018 May 27

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Silicate Cement
Calcium silicate
Cements
Minerals
Osteocalcin
calcium silicate
mineral trioxide aggregate
Pulp

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Kang, Chung Min ; Hwang, Jiwon ; Song, Je Seon ; Lee, Jae Ho ; Choi, Hyung Jun ; Shin, Yooseok. / Effects of three calcium silicate cements on inflammatory response and mineralization-inducing potentials in a dog pulpotomy model. In: Materials. 2018 ; Vol. 11, No. 6.
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abstract = "This beagle pulpotomy study compared the inflammatory response and mineralizationinducing potential of three calcium silicate cements: ProRoot mineral trioxide aggregate (MTA) (Dentsply, Tulsa, OK, USA), OrthoMTA (BioMTA, Seoul, Korea), and Endocem MTA (Maruchi, Wonju, Korea). Exposed pulp tissues were capped with ProRoot MTA, OrthoMTA, or Endocem MTA. After 8 weeks, we extracted the teeth, then performed hematoxylin-eosin and immunohistochemical staining with osteocalcin and dentin sialoprotein. Histological evaluation comprised a scoring system with eight broad categories and analysis of calcific barrier areas. We evaluated 44 teeth capped with ProRoot MTA (n = 15), OrthoMTA (n = 18), or Endocem MTA (n = 11). Most ProRoot MTA specimens formed continuous calcific barriers; these pulps contained inflammation-free palisading patterns in the odontoblastic layer. Areas of the newly formed calcific barrier were greater with ProRoot MTA than with Endocem MTA (p = 0.006). Although dentin sialoprotein was highly expressed in all three groups, the osteocalcin expression was reduced in the OrthoMTA and Endocem MTA groups. ProRoot MTA was superior to OrthoMTA and Endocem MTA in all histological analyses. ProRoot MTA and OrthoMTA resulted in reduced pulpal inflammation and more complete calcific barrier formation, whereas Endocem MTA caused a lower level of calcific barrier continuity with tunnel defects.",
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Effects of three calcium silicate cements on inflammatory response and mineralization-inducing potentials in a dog pulpotomy model. / Kang, Chung Min; Hwang, Jiwon; Song, Je Seon; Lee, Jae Ho; Choi, Hyung Jun; Shin, Yooseok.

In: Materials, Vol. 11, No. 6, 899, 27.05.2018.

Research output: Contribution to journalArticle

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