Effects of various dilute acid pretreatments on the biochemical hydrogen production potential of marine macroalgal biomass

Periyasamy Sivagurunathan, Gopalakrishnan Kumar, Takuro Kobayashi, Kaiqin Xu, Sang Hyoun Kim

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40 Citations (Scopus)


This research investigated the effects of various dilute acid (HCl, H2SO4, HNO3, H3PO4) pretreatment on the solubilization of marine macroalgal biomass Gelidium amansii and subsequent hydrogen fermentation in a batch vials. The dry grounded biomass was hydrolyzed at temperature of 121 °C, solid/liquid (S/L) ratio of 5% (w/v), dilute acid concentration (1%) of various reagents, and reaction time of 30 min. The hydrolyzates obtained at these conditions were then fed to batch hydrogen fermentation. Results revealed that only H2SO4 pretreatment method had a significant effect on improvement of biohydrogen production from Gelidium amansii, whereas the other pretreatment conditions were even worse than the control experiment. Dilute sulfuric acid (1%) provided a maximum hydrogen production of 52 mL-H2/g-dry biomass, whereas control experiment provided a hydrogen efficiency of 27 mL-H2/g-dry biomass. The results showed that selection of appropriate pretreatment method is essential for enhanced hydrogen production from macroalgal biomass.

Original languageEnglish
Pages (from-to)27600-27606
Number of pages7
JournalInternational Journal of Hydrogen Energy
Issue number45
Publication statusPublished - 2017

Bibliographical note

Funding Information:
The author Dr. Periyasamy Sivagurunathan greatly acknowledged the financial assistance from Japan Society for Promotion of Science : JSPS ID: P15370 for this study. The financial assistance to one of the authors (GK) from Ton Duc Thang University, Vietnam is highly acknowledged.

Publisher Copyright:
© 2017 Hydrogen Energy Publications LLC

All Science Journal Classification (ASJC) codes

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology


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