Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay

Go Eun Choi; Chulhun L. Chang; Jake Whang; Hwa Jung Kim; O. Jung Kwon; Won Jung Koh; Sung Jae Shin

doi:10.1128/JCM.02318-10

Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay

Go Eun Choi, Chulhun L. Chang, Jake Whang, Hwa Jung Kim, O. Jung Kwon, Won Jung Koh, Sung Jae Shin

Department of Microbiology

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.

Original language	English
Pages (from-to)	1107-1109
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	3
DOIs	https://doi.org/10.1128/JCM.02318-10
Publication status	Published - 2011 Mar

All Science Journal Classification (ASJC) codes

Microbiology (medical)

Access to Document

10.1128/JCM.02318-10

Cite this

@article{ee9814008e044e658d0d3624941444e0,

title = "Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay",

abstract = "A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.",

author = "Choi, {Go Eun} and Chang, {Chulhun L.} and Jake Whang and Kim, {Hwa Jung} and Kwon, {O. Jung} and Koh, {Won Jung} and Shin, {Sung Jae}",

year = "2011",

month = mar,

doi = "10.1128/JCM.02318-10",

language = "English",

volume = "49",

pages = "1107--1109",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "3",

}

Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay. / Choi, Go Eun; Chang, Chulhun L.; Whang, Jake; Kim, Hwa Jung; Kwon, O. Jung; Koh, Won Jung; Shin, Sung Jae.

In: Journal of Clinical Microbiology, Vol. 49, No. 3, 03.2011, p. 1107-1109.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay

AU - Choi, Go Eun

AU - Chang, Chulhun L.

AU - Whang, Jake

AU - Kim, Hwa Jung

AU - Kwon, O. Jung

AU - Koh, Won Jung

AU - Shin, Sung Jae

PY - 2011/3

Y1 - 2011/3

N2 - A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.

AB - A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.

UR - http://www.scopus.com/inward/record.url?scp=79952339073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952339073&partnerID=8YFLogxK

U2 - 10.1128/JCM.02318-10

DO - 10.1128/JCM.02318-10

M3 - Article

C2 - 21177894

AN - SCOPUS:79952339073

VL - 49

SP - 1107

EP - 1109

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

ER -

Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this