Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay

Go Eun Choi, Chulhun L. Chang, Jake Whang, Hwa Jung Kim, O. Jung Kwon, Won Jung Koh, Sung Jae Shin

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.

Original languageEnglish
Pages (from-to)1107-1109
Number of pages3
JournalJournal of Clinical Microbiology
Volume49
Issue number3
DOIs
Publication statusPublished - 2011 Mar

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

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