Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay

Go Eun Choi; Chulhun L. Chang; Jake Whang; Hwa Jung Kim; O. Jung Kwon; Won Jung Koh; Sung Jae Shin

doi:10.1128/JCM.02318-10

Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay

Go Eun Choi, Chulhun L. Chang, Jake Whang, Hwa Jung Kim, O. Jung Kwon, Won Jung Koh, Sung Jae Shin

Department of Microbiology

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.

Original language	English
Pages (from-to)	1107-1109
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	49
Issue number	3
DOIs	https://doi.org/10.1128/JCM.02318-10
Publication status	Published - 2011 Mar

All Science Journal Classification (ASJC) codes

Microbiology (medical)

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10.1128/JCM.02318-10

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abstract = "A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.",

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AU - Kim, Hwa Jung

AU - Kwon, O. Jung

AU - Koh, Won Jung

AU - Shin, Sung Jae

PY - 2011/3

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AB - A novel duplex PCR method based on variable-number tandem-repeat targets to discriminate among Mycobacterium abscessus complex isolates was developed and evaluated in 85 clinical isolates. The assay accuracy was confirmed by a multiple-target sequence analysis. The duplex PCR assay is a one-step, reliable, and accurate assay for discriminating M. abscessus species.

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Efficient differentiation of Mycobacterium abscessus complex isolates to the species level by a novel PCR-based variable-number tandem-repeat assay

Abstract

All Science Journal Classification (ASJC) codes

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