Efficient method for extraction and simultaneous determination of active constituents in Cornus officinalis by reflux extraction and high performance liquid chromatography with diode array detection

Seung Hyun Kim, Mi Kyeong Lee, Mi Jeong Ahn, Ki Yong Lee, Young Choong Kim, Sang Hyun Sung

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A high performance liquid chromatography diode array detector (HPLC-DAD) based method was developed for the simultaneous determination of the three active constituents, morroniside, loganin, and gallic acid in Cornus officinalis fruits. The separation was achieved on a C18 column with a gradient solvent system of acetonitrile, methanol, and 0.1% acetic acid at 260 nm. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The analytes were confirmed by electrospray ionization mass spectrometry (ESI-MS). In addition, the effect of various parameters including solvent, extraction technique, and time on the extraction of the three active constituents from C. officinalis fruits was examined. The highest extraction efficiency was obtained with 100% methanol using reflux extraction. The developed method could be used to effectively and comprehensively evaluate the quality of C. officinalis.

Original languageEnglish
Pages (from-to)822-832
Number of pages11
JournalJournal of Liquid Chromatography and Related Technologies
Volume32
Issue number6
DOIs
Publication statusPublished - 2009 Jan 1

Fingerprint

Cornus
High performance liquid chromatography
Diodes
High Pressure Liquid Chromatography
Fruits
Methanol
Fruit
Electrospray ionization
Gallic Acid
Electrospray Ionization Mass Spectrometry
Solvent extraction
Acetic Acid
Mass spectrometry
Limit of Detection
Detectors

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

Cite this

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title = "Efficient method for extraction and simultaneous determination of active constituents in Cornus officinalis by reflux extraction and high performance liquid chromatography with diode array detection",
abstract = "A high performance liquid chromatography diode array detector (HPLC-DAD) based method was developed for the simultaneous determination of the three active constituents, morroniside, loganin, and gallic acid in Cornus officinalis fruits. The separation was achieved on a C18 column with a gradient solvent system of acetonitrile, methanol, and 0.1{\%} acetic acid at 260 nm. The method was validated for linearity, repeatability, limits of detection, and limits of quantification. The analytes were confirmed by electrospray ionization mass spectrometry (ESI-MS). In addition, the effect of various parameters including solvent, extraction technique, and time on the extraction of the three active constituents from C. officinalis fruits was examined. The highest extraction efficiency was obtained with 100{\%} methanol using reflux extraction. The developed method could be used to effectively and comprehensively evaluate the quality of C. officinalis.",
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Efficient method for extraction and simultaneous determination of active constituents in Cornus officinalis by reflux extraction and high performance liquid chromatography with diode array detection. / Kim, Seung Hyun; Lee, Mi Kyeong; Ahn, Mi Jeong; Lee, Ki Yong; Kim, Young Choong; Sung, Sang Hyun.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 32, No. 6, 01.01.2009, p. 822-832.

Research output: Contribution to journalArticle

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