Eight-week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs

Ji Sook Park, Seongho Choi, Ik Sang Moon, Kyoo Sung Cho, Jung Kiu Chai, Chong Kwan Kim

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Objective: To evaluate the periodontal tissue regenerative effects of a chitosan/ collagen sponge applied to preclinical one-wall intrabony defects surgically created in beagle dogs. Material and Methods: 4 x 4 mm one-wall intrabony defects were surgically created in the bilateral maxillary first and third, and the mandibular second and fourth premolars. The surgical control group received a flap operation only, while the buffer control group was treated afterwards with a phosphate-buffered saline/collagen sponge (CS) and the chitosan group was treated with a chitosan/cs. The subjects were killed 8 weeks after the operation, and a comparative histological examination was performed. Results: The amount of junctional epithelium migration was 2.30 ± 1.24 mm in the surgical control group, 1.49 ± 1.25 mm in the buffer control group, and 0.26 ± 0.59 mm in the chitosan group. A significant difference was exhibited only between the surgical control and the chitosan group (p < 0.05). The amount of connective tissue adhesion was 0.68 ± 0.60, 1.07 ± 0.91, and 0.41 ± 0.42 mm in the surgical control, buffer control, and the chitosan group, respectively. The amount of cementum regeneration was 1.42 ± 0.49, 1.60 ± 0.41, and 3.46 ± 0.78 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was seen between the chitosan group and the rest (p < 0.01). The amount of alveolar bone regeneration was 1.00 ± 0.77, 1.52 ± 0.37, and 2.43 ± 0.44 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was observed between the chitosan group and the rest (p < 0.05). Conclusion: The results demonstrate the beneficial effect of the chitosan/cs on the one-wall intrabony defects of beagle dogs. The inhibited apical migration of epithelium and the increase in the amount of new bone and new cementum suggest the potency of chitosan in inducing periodontal tissue regeneration.

Original languageEnglish
Pages (from-to)443-453
Number of pages11
JournalJournal of Clinical Periodontology
Volume30
Issue number5
DOIs
Publication statusPublished - 2003 May 1

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Chitosan
Dogs
Control Groups
Buffers
Dental Cementum
Porifera
Regeneration
Collagen
Epithelial Attachment
Tissue Adhesions
Bone Regeneration
Bicuspid
Connective Tissue
Epithelium
Phosphates

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Park, Ji Sook ; Choi, Seongho ; Moon, Ik Sang ; Cho, Kyoo Sung ; Chai, Jung Kiu ; Kim, Chong Kwan. / Eight-week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs. In: Journal of Clinical Periodontology. 2003 ; Vol. 30, No. 5. pp. 443-453.
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title = "Eight-week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs",
abstract = "Objective: To evaluate the periodontal tissue regenerative effects of a chitosan/ collagen sponge applied to preclinical one-wall intrabony defects surgically created in beagle dogs. Material and Methods: 4 x 4 mm one-wall intrabony defects were surgically created in the bilateral maxillary first and third, and the mandibular second and fourth premolars. The surgical control group received a flap operation only, while the buffer control group was treated afterwards with a phosphate-buffered saline/collagen sponge (CS) and the chitosan group was treated with a chitosan/cs. The subjects were killed 8 weeks after the operation, and a comparative histological examination was performed. Results: The amount of junctional epithelium migration was 2.30 ± 1.24 mm in the surgical control group, 1.49 ± 1.25 mm in the buffer control group, and 0.26 ± 0.59 mm in the chitosan group. A significant difference was exhibited only between the surgical control and the chitosan group (p < 0.05). The amount of connective tissue adhesion was 0.68 ± 0.60, 1.07 ± 0.91, and 0.41 ± 0.42 mm in the surgical control, buffer control, and the chitosan group, respectively. The amount of cementum regeneration was 1.42 ± 0.49, 1.60 ± 0.41, and 3.46 ± 0.78 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was seen between the chitosan group and the rest (p < 0.01). The amount of alveolar bone regeneration was 1.00 ± 0.77, 1.52 ± 0.37, and 2.43 ± 0.44 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was observed between the chitosan group and the rest (p < 0.05). Conclusion: The results demonstrate the beneficial effect of the chitosan/cs on the one-wall intrabony defects of beagle dogs. The inhibited apical migration of epithelium and the increase in the amount of new bone and new cementum suggest the potency of chitosan in inducing periodontal tissue regeneration.",
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Eight-week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs. / Park, Ji Sook; Choi, Seongho; Moon, Ik Sang; Cho, Kyoo Sung; Chai, Jung Kiu; Kim, Chong Kwan.

In: Journal of Clinical Periodontology, Vol. 30, No. 5, 01.05.2003, p. 443-453.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Eight-week histological analysis on the effect of chitosan on surgically created one-wall intrabony defects in beagle dogs

AU - Park, Ji Sook

AU - Choi, Seongho

AU - Moon, Ik Sang

AU - Cho, Kyoo Sung

AU - Chai, Jung Kiu

AU - Kim, Chong Kwan

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N2 - Objective: To evaluate the periodontal tissue regenerative effects of a chitosan/ collagen sponge applied to preclinical one-wall intrabony defects surgically created in beagle dogs. Material and Methods: 4 x 4 mm one-wall intrabony defects were surgically created in the bilateral maxillary first and third, and the mandibular second and fourth premolars. The surgical control group received a flap operation only, while the buffer control group was treated afterwards with a phosphate-buffered saline/collagen sponge (CS) and the chitosan group was treated with a chitosan/cs. The subjects were killed 8 weeks after the operation, and a comparative histological examination was performed. Results: The amount of junctional epithelium migration was 2.30 ± 1.24 mm in the surgical control group, 1.49 ± 1.25 mm in the buffer control group, and 0.26 ± 0.59 mm in the chitosan group. A significant difference was exhibited only between the surgical control and the chitosan group (p < 0.05). The amount of connective tissue adhesion was 0.68 ± 0.60, 1.07 ± 0.91, and 0.41 ± 0.42 mm in the surgical control, buffer control, and the chitosan group, respectively. The amount of cementum regeneration was 1.42 ± 0.49, 1.60 ± 0.41, and 3.46 ± 0.78 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was seen between the chitosan group and the rest (p < 0.01). The amount of alveolar bone regeneration was 1.00 ± 0.77, 1.52 ± 0.37, and 2.43 ± 0.44 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was observed between the chitosan group and the rest (p < 0.05). Conclusion: The results demonstrate the beneficial effect of the chitosan/cs on the one-wall intrabony defects of beagle dogs. The inhibited apical migration of epithelium and the increase in the amount of new bone and new cementum suggest the potency of chitosan in inducing periodontal tissue regeneration.

AB - Objective: To evaluate the periodontal tissue regenerative effects of a chitosan/ collagen sponge applied to preclinical one-wall intrabony defects surgically created in beagle dogs. Material and Methods: 4 x 4 mm one-wall intrabony defects were surgically created in the bilateral maxillary first and third, and the mandibular second and fourth premolars. The surgical control group received a flap operation only, while the buffer control group was treated afterwards with a phosphate-buffered saline/collagen sponge (CS) and the chitosan group was treated with a chitosan/cs. The subjects were killed 8 weeks after the operation, and a comparative histological examination was performed. Results: The amount of junctional epithelium migration was 2.30 ± 1.24 mm in the surgical control group, 1.49 ± 1.25 mm in the buffer control group, and 0.26 ± 0.59 mm in the chitosan group. A significant difference was exhibited only between the surgical control and the chitosan group (p < 0.05). The amount of connective tissue adhesion was 0.68 ± 0.60, 1.07 ± 0.91, and 0.41 ± 0.42 mm in the surgical control, buffer control, and the chitosan group, respectively. The amount of cementum regeneration was 1.42 ± 0.49, 1.60 ± 0.41, and 3.46 ± 0.78 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was seen between the chitosan group and the rest (p < 0.01). The amount of alveolar bone regeneration was 1.00 ± 0.77, 1.52 ± 0.37, and 2.43 ± 0.44 mm in the surgical control, buffer control, and the chitosan group, respectively. A significant difference was observed between the chitosan group and the rest (p < 0.05). Conclusion: The results demonstrate the beneficial effect of the chitosan/cs on the one-wall intrabony defects of beagle dogs. The inhibited apical migration of epithelium and the increase in the amount of new bone and new cementum suggest the potency of chitosan in inducing periodontal tissue regeneration.

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