We developed entangled link-augmented stretchable tissue-hydrogel (ELAST), a technology that transforms tissues into elastic hydrogels to enhance macromolecular accessibility and mechanical stability simultaneously. ELASTicized tissues are highly stretchable and compressible, which enables reversible shape transformation and faster delivery of probes into intact tissue specimens via mechanical thinning. This universal platform may facilitate rapid and scalable molecular phenotyping of large-scale biological systems, such as human organs.
Bibliographical noteFunding Information:
We thank the entire Chung Laboratory for support and discussions. We thank S.-C. Chen and J. Wang for the development of the vibrating-blade microtome, and D. H. Yun for its operation. We also thank K. Xie for mouse sample preparation, Y. Tian for the initial diffusion modeling work and R. Thorndike-Breeze for comments on improving the manuscript. We thank H. Jung and H. Kim for providing the histology facility and helping the H&E experiment. K.C. was supported by the Burroughs Wellcome Fund Career Awards at the Scientific Interface, the Searle Scholars Program, the Packard Award in Science and Engineering, the NARSAD Young Investigator Award, the Institute for Basic Science grant no. IBS-R026-D1 and the McKnight Foundation Technology Award. W.G. was supported by the National Science Foundation Graduate Research Fellowship under grant no. 1122374. A.A. was supported by the JPB Foundation (Picower Fellowship). M.P.F. was partially supported by NIA grant no. P50 AG005134. This work was supported by the JPB Foundation (grant nos. PIIF and PNDRF), the NCSOFT Cultural Foundation, and the NIH (grant nos. 1-DP2-ES027992, U01MH117072).
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology