Elasticizing tissues for reversible shape transformation and accelerated molecular labeling

Taeyun Ku; Webster Guan; Nicholas B. Evans; Chang Ho Sohn; Alexandre Albanese; Joon Goon Kim; Matthew P. Frosch; Kwanghun Chung

doi:10.1038/s41592-020-0823-y

Elasticizing tissues for reversible shape transformation and accelerated molecular labeling

Taeyun Ku, Webster Guan, Nicholas B. Evans, Chang Ho Sohn, Alexandre Albanese, Joon Goon Kim, Matthew P. Frosch, Kwanghun Chung

Yonsei Advanced Science Institute

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We developed entangled link-augmented stretchable tissue-hydrogel (ELAST), a technology that transforms tissues into elastic hydrogels to enhance macromolecular accessibility and mechanical stability simultaneously. ELASTicized tissues are highly stretchable and compressible, which enables reversible shape transformation and faster delivery of probes into intact tissue specimens via mechanical thinning. This universal platform may facilitate rapid and scalable molecular phenotyping of large-scale biological systems, such as human organs.

Original language	English
Pages (from-to)	609-613
Number of pages	5
Journal	Nature Methods
Volume	17
Issue number	6
DOIs	https://doi.org/10.1038/s41592-020-0823-y
Publication status	Published - 2020 Jun 1

Bibliographical note

Funding Information:
We thank the entire Chung Laboratory for support and discussions. We thank S.-C. Chen and J. Wang for the development of the vibrating-blade microtome, and D. H. Yun for its operation. We also thank K. Xie for mouse sample preparation, Y. Tian for the initial diffusion modeling work and R. Thorndike-Breeze for comments on improving the manuscript. We thank H. Jung and H. Kim for providing the histology facility and helping the H&E experiment. K.C. was supported by the Burroughs Wellcome Fund Career Awards at the Scientific Interface, the Searle Scholars Program, the Packard Award in Science and Engineering, the NARSAD Young Investigator Award, the Institute for Basic Science grant no. IBS-R026-D1 and the McKnight Foundation Technology Award. W.G. was supported by the National Science Foundation Graduate Research Fellowship under grant no. 1122374. A.A. was supported by the JPB Foundation (Picower Fellowship). M.P.F. was partially supported by NIA grant no. P50 AG005134. This work was supported by the JPB Foundation (grant nos. PIIF and PNDRF), the NCSOFT Cultural Foundation, and the NIH (grant nos. 1-DP2-ES027992, U01MH117072).

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1038/s41592-020-0823-y

Fingerprint Dive into the research topics of 'Elasticizing tissues for reversible shape transformation and accelerated molecular labeling'. Together they form a unique fingerprint.

Cite this

@article{d83e4f4f67f64c3282fefd4f224bb2d4,

title = "Elasticizing tissues for reversible shape transformation and accelerated molecular labeling",

abstract = "We developed entangled link-augmented stretchable tissue-hydrogel (ELAST), a technology that transforms tissues into elastic hydrogels to enhance macromolecular accessibility and mechanical stability simultaneously. ELASTicized tissues are highly stretchable and compressible, which enables reversible shape transformation and faster delivery of probes into intact tissue specimens via mechanical thinning. This universal platform may facilitate rapid and scalable molecular phenotyping of large-scale biological systems, such as human organs.",

author = "Taeyun Ku and Webster Guan and Evans, {Nicholas B.} and Sohn, {Chang Ho} and Alexandre Albanese and Kim, {Joon Goon} and Frosch, {Matthew P.} and Kwanghun Chung",

note = "Funding Information: We thank the entire Chung Laboratory for support and discussions. We thank S.-C. Chen and J. Wang for the development of the vibrating-blade microtome, and D. H. Yun for its operation. We also thank K. Xie for mouse sample preparation, Y. Tian for the initial diffusion modeling work and R. Thorndike-Breeze for comments on improving the manuscript. We thank H. Jung and H. Kim for providing the histology facility and helping the H&E experiment. K.C. was supported by the Burroughs Wellcome Fund Career Awards at the Scientific Interface, the Searle Scholars Program, the Packard Award in Science and Engineering, the NARSAD Young Investigator Award, the Institute for Basic Science grant no. IBS-R026-D1 and the McKnight Foundation Technology Award. W.G. was supported by the National Science Foundation Graduate Research Fellowship under grant no. 1122374. A.A. was supported by the JPB Foundation (Picower Fellowship). M.P.F. was partially supported by NIA grant no. P50 AG005134. This work was supported by the JPB Foundation (grant nos. PIIF and PNDRF), the NCSOFT Cultural Foundation, and the NIH (grant nos. 1-DP2-ES027992, U01MH117072).",

year = "2020",

month = jun,

day = "1",

doi = "10.1038/s41592-020-0823-y",

language = "English",

volume = "17",

pages = "609--613",

journal = "Nature Methods",

issn = "1548-7091",

publisher = "Nature Publishing Group",

number = "6",

}

TY - JOUR

T1 - Elasticizing tissues for reversible shape transformation and accelerated molecular labeling

AU - Ku, Taeyun

AU - Guan, Webster

AU - Evans, Nicholas B.

AU - Sohn, Chang Ho

AU - Albanese, Alexandre

AU - Kim, Joon Goon

AU - Frosch, Matthew P.

AU - Chung, Kwanghun

N1 - Funding Information: We thank the entire Chung Laboratory for support and discussions. We thank S.-C. Chen and J. Wang for the development of the vibrating-blade microtome, and D. H. Yun for its operation. We also thank K. Xie for mouse sample preparation, Y. Tian for the initial diffusion modeling work and R. Thorndike-Breeze for comments on improving the manuscript. We thank H. Jung and H. Kim for providing the histology facility and helping the H&E experiment. K.C. was supported by the Burroughs Wellcome Fund Career Awards at the Scientific Interface, the Searle Scholars Program, the Packard Award in Science and Engineering, the NARSAD Young Investigator Award, the Institute for Basic Science grant no. IBS-R026-D1 and the McKnight Foundation Technology Award. W.G. was supported by the National Science Foundation Graduate Research Fellowship under grant no. 1122374. A.A. was supported by the JPB Foundation (Picower Fellowship). M.P.F. was partially supported by NIA grant no. P50 AG005134. This work was supported by the JPB Foundation (grant nos. PIIF and PNDRF), the NCSOFT Cultural Foundation, and the NIH (grant nos. 1-DP2-ES027992, U01MH117072).

PY - 2020/6/1

Y1 - 2020/6/1

N2 - We developed entangled link-augmented stretchable tissue-hydrogel (ELAST), a technology that transforms tissues into elastic hydrogels to enhance macromolecular accessibility and mechanical stability simultaneously. ELASTicized tissues are highly stretchable and compressible, which enables reversible shape transformation and faster delivery of probes into intact tissue specimens via mechanical thinning. This universal platform may facilitate rapid and scalable molecular phenotyping of large-scale biological systems, such as human organs.

AB - We developed entangled link-augmented stretchable tissue-hydrogel (ELAST), a technology that transforms tissues into elastic hydrogels to enhance macromolecular accessibility and mechanical stability simultaneously. ELASTicized tissues are highly stretchable and compressible, which enables reversible shape transformation and faster delivery of probes into intact tissue specimens via mechanical thinning. This universal platform may facilitate rapid and scalable molecular phenotyping of large-scale biological systems, such as human organs.

UR - http://www.scopus.com/inward/record.url?scp=85084817058&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85084817058&partnerID=8YFLogxK

U2 - 10.1038/s41592-020-0823-y

DO - 10.1038/s41592-020-0823-y

M3 - Article

C2 - 32424271

AN - SCOPUS:85084817058

VL - 17

SP - 609

EP - 613

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 6

ER -