Electrical antimicrobial susceptibility testing based on aptamer-functionalized capacitance sensor array for clinical isolates

Kyo Seok Lee, Sun Mi Lee, Jeseung Oh, In Ho Park, Jun Ho Song, Myeonggil Han, Dongeun Yong, Kook Jin Lim, Jeon Soo Shin, Kyung Hwa Yoo

Research output: Contribution to journalArticle

Abstract

To prescribe effective antibiotics to patients with bacterial infections in a timely manner and to avoid the misuse of antibiotics, a rapid antimicrobial susceptibility test (AST) is essential. However, conventional AST methods require more than 16 h to provide results; thus, we developed an electrical AST (e-AST) system, which provides results within 6 h. The proposed e-AST is based on an array of 60 aptamer-functionalized capacitance sensors that are comparable to currently available AST panels and a pattern-matching algorithm. The performance of the e-AST was evaluated in comparison with that of broth microdilution as the reference test for clinical strains isolated from septic patients. A total of 4,554 tests using e-AST showed a categorical agreement of 97% with a minor error of 2.2%, major error of 0.38%, and very major error of 0.38%. We expect that the proposed e-AST could potentially aid antimicrobial stewardship efforts and lead to improved patient outcomes.

Original languageEnglish
Article number13709
JournalScientific reports
Volume10
Issue number1
DOIs
Publication statusPublished - 2020 Dec 1

All Science Journal Classification (ASJC) codes

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