Electrochemical determination of carbohydrate-binding proteins using carbohydrate-stabilized gold nanoparticles and silver enhancement

In Hong Min, Lee Choi, Kwang Soo Ahn, Byung Kun Kim, Bo Young Lee, Kwan Soo Kim, Han Nim Choi, Won Yong Lee

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

A highly sensitive electrochemical lectin biosensor has been developed for the first time using carbohydrate-stabilized gold nanoparticles and silver-enhancement technique. A target lectin protein, Concanavalin A (Con A), was specifically bound to the self-assembled monolayer of thiolated mannose on a gold electrode. Mannose-stabilized gold nanoparticles were added to form a sandwich-type complex with the Con A and were followed by silver-enhancement process to coat the mannose-stabilized gold nanoparticles with silver metal. The coated metallic silver was dissolved in an acidic solution and the resulting silver ions were detected by anodic stripping voltammetry. The present lectin biosensor gave a linear response (R2=0.999) for Con A concentration from 0.084μg/mL to 50.0μg/mL with a remarkable detection limit (S/N=3) of 0.070μg/mL, which is much lower compared to those obtained with the reported microgravimetric and colorimetric detection methods.

Original languageEnglish
Pages (from-to)1326-1331
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number4
DOIs
Publication statusPublished - 2010 Dec 15

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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