Electrochemical determination of carbohydrate-binding proteins using carbohydrate-stabilized gold nanoparticles and silver enhancement

In Hong Min, Lee Choi, Kwang Soo Ahn, Byung Kun Kim, Bo Young Lee, Kwan Soo Kim, Han Nim Choi, Won Yong Lee

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

A highly sensitive electrochemical lectin biosensor has been developed for the first time using carbohydrate-stabilized gold nanoparticles and silver-enhancement technique. A target lectin protein, Concanavalin A (Con A), was specifically bound to the self-assembled monolayer of thiolated mannose on a gold electrode. Mannose-stabilized gold nanoparticles were added to form a sandwich-type complex with the Con A and were followed by silver-enhancement process to coat the mannose-stabilized gold nanoparticles with silver metal. The coated metallic silver was dissolved in an acidic solution and the resulting silver ions were detected by anodic stripping voltammetry. The present lectin biosensor gave a linear response (R2=0.999) for Con A concentration from 0.084μg/mL to 50.0μg/mL with a remarkable detection limit (S/N=3) of 0.070μg/mL, which is much lower compared to those obtained with the reported microgravimetric and colorimetric detection methods.

Original languageEnglish
Pages (from-to)1326-1331
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number4
DOIs
Publication statusPublished - 2010 Dec 15

Fingerprint

Carbohydrates
Silver
Gold
Nanoparticles
Mannose
Concanavalin A
Lectins
Biosensing Techniques
Biosensors
Self assembled monolayers
Voltammetry
Limit of Detection
Electrodes
Metals
saccharide-binding proteins
Carrier Proteins
Ions
Proteins

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

Min, In Hong ; Choi, Lee ; Ahn, Kwang Soo ; Kim, Byung Kun ; Lee, Bo Young ; Kim, Kwan Soo ; Choi, Han Nim ; Lee, Won Yong. / Electrochemical determination of carbohydrate-binding proteins using carbohydrate-stabilized gold nanoparticles and silver enhancement. In: Biosensors and Bioelectronics. 2010 ; Vol. 26, No. 4. pp. 1326-1331.
@article{72c9295168e945e0bd373fc0b044b22b,
title = "Electrochemical determination of carbohydrate-binding proteins using carbohydrate-stabilized gold nanoparticles and silver enhancement",
abstract = "A highly sensitive electrochemical lectin biosensor has been developed for the first time using carbohydrate-stabilized gold nanoparticles and silver-enhancement technique. A target lectin protein, Concanavalin A (Con A), was specifically bound to the self-assembled monolayer of thiolated mannose on a gold electrode. Mannose-stabilized gold nanoparticles were added to form a sandwich-type complex with the Con A and were followed by silver-enhancement process to coat the mannose-stabilized gold nanoparticles with silver metal. The coated metallic silver was dissolved in an acidic solution and the resulting silver ions were detected by anodic stripping voltammetry. The present lectin biosensor gave a linear response (R2=0.999) for Con A concentration from 0.084μg/mL to 50.0μg/mL with a remarkable detection limit (S/N=3) of 0.070μg/mL, which is much lower compared to those obtained with the reported microgravimetric and colorimetric detection methods.",
author = "Min, {In Hong} and Lee Choi and Ahn, {Kwang Soo} and Kim, {Byung Kun} and Lee, {Bo Young} and Kim, {Kwan Soo} and Choi, {Han Nim} and Lee, {Won Yong}",
year = "2010",
month = "12",
day = "15",
doi = "10.1016/j.bios.2010.07.038",
language = "English",
volume = "26",
pages = "1326--1331",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Limited",
number = "4",

}

Electrochemical determination of carbohydrate-binding proteins using carbohydrate-stabilized gold nanoparticles and silver enhancement. / Min, In Hong; Choi, Lee; Ahn, Kwang Soo; Kim, Byung Kun; Lee, Bo Young; Kim, Kwan Soo; Choi, Han Nim; Lee, Won Yong.

In: Biosensors and Bioelectronics, Vol. 26, No. 4, 15.12.2010, p. 1326-1331.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Electrochemical determination of carbohydrate-binding proteins using carbohydrate-stabilized gold nanoparticles and silver enhancement

AU - Min, In Hong

AU - Choi, Lee

AU - Ahn, Kwang Soo

AU - Kim, Byung Kun

AU - Lee, Bo Young

AU - Kim, Kwan Soo

AU - Choi, Han Nim

AU - Lee, Won Yong

PY - 2010/12/15

Y1 - 2010/12/15

N2 - A highly sensitive electrochemical lectin biosensor has been developed for the first time using carbohydrate-stabilized gold nanoparticles and silver-enhancement technique. A target lectin protein, Concanavalin A (Con A), was specifically bound to the self-assembled monolayer of thiolated mannose on a gold electrode. Mannose-stabilized gold nanoparticles were added to form a sandwich-type complex with the Con A and were followed by silver-enhancement process to coat the mannose-stabilized gold nanoparticles with silver metal. The coated metallic silver was dissolved in an acidic solution and the resulting silver ions were detected by anodic stripping voltammetry. The present lectin biosensor gave a linear response (R2=0.999) for Con A concentration from 0.084μg/mL to 50.0μg/mL with a remarkable detection limit (S/N=3) of 0.070μg/mL, which is much lower compared to those obtained with the reported microgravimetric and colorimetric detection methods.

AB - A highly sensitive electrochemical lectin biosensor has been developed for the first time using carbohydrate-stabilized gold nanoparticles and silver-enhancement technique. A target lectin protein, Concanavalin A (Con A), was specifically bound to the self-assembled monolayer of thiolated mannose on a gold electrode. Mannose-stabilized gold nanoparticles were added to form a sandwich-type complex with the Con A and were followed by silver-enhancement process to coat the mannose-stabilized gold nanoparticles with silver metal. The coated metallic silver was dissolved in an acidic solution and the resulting silver ions were detected by anodic stripping voltammetry. The present lectin biosensor gave a linear response (R2=0.999) for Con A concentration from 0.084μg/mL to 50.0μg/mL with a remarkable detection limit (S/N=3) of 0.070μg/mL, which is much lower compared to those obtained with the reported microgravimetric and colorimetric detection methods.

UR - http://www.scopus.com/inward/record.url?scp=78649735216&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=78649735216&partnerID=8YFLogxK

U2 - 10.1016/j.bios.2010.07.038

DO - 10.1016/j.bios.2010.07.038

M3 - Article

C2 - 20685103

AN - SCOPUS:78649735216

VL - 26

SP - 1326

EP - 1331

JO - Biosensors and Bioelectronics

JF - Biosensors and Bioelectronics

SN - 0956-5663

IS - 4

ER -