Electrochemical ELISA based on E. coli with autodisplayed Z-domains

Ju Kyung Lee, Gu-Yoo, Park Min, Joachim Jose, Jae Chul Pyun

Research output: Contribution to journalConference articlepeer-review

Abstract

Recently, we reported autodisplayed Z-domains on E.coli can highly improve the sensitivity of immunoassays through the orientation control of detection antibodies [1-3]. In this work, the amperometric analysis was applied to the immunoassay based on E.coli cells with autodisplayed Z-domains. For amperometric analaysis, the reduction current of 3,5,3′,5′-tetramethylbenzidine (TMB) was measured at the potential of -50 mV and the correlation between the reduction current and the OD value was estimated to be 28 nA/OD and the limit of detection was calculated to be 0.07 in OD unit. In this work, an immunoassay was prepared by immobilizing anti-HRP antibodies to E.coli cells with autodisplayed Z-domains and the amperometric analysis was demonstrated the quantification of bound HRP. For the demonstration of medical diagnosis, C-reactive protein (CRP) was detected by using electrochemical ELISA based on E.coli with autodisplayed Z-domains.

Original languageEnglish
Pages (from-to)944-947
Number of pages4
JournalProcedia Engineering
Volume25
DOIs
Publication statusPublished - 2011
Event25th Eurosensors Conference - Athens, Greece
Duration: 2011 Sep 42011 Sep 7

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0082188, 2009-0073809, 2009-008-1529, 2010-0020767 and 2010-0020772).

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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