Abstract
Recently, we reported autodisplayed Z-domains on E.coli can highly improve the sensitivity of immunoassays through the orientation control of detection antibodies [1-3]. In this work, the amperometric analysis was applied to the immunoassay based on E.coli cells with autodisplayed Z-domains. For amperometric analaysis, the reduction current of 3,5,3′,5′-tetramethylbenzidine (TMB) was measured at the potential of -50 mV and the correlation between the reduction current and the OD value was estimated to be 28 nA/OD and the limit of detection was calculated to be 0.07 in OD unit. In this work, an immunoassay was prepared by immobilizing anti-HRP antibodies to E.coli cells with autodisplayed Z-domains and the amperometric analysis was demonstrated the quantification of bound HRP. For the demonstration of medical diagnosis, C-reactive protein (CRP) was detected by using electrochemical ELISA based on E.coli with autodisplayed Z-domains.
Original language | English |
---|---|
Pages (from-to) | 944-947 |
Number of pages | 4 |
Journal | Procedia Engineering |
Volume | 25 |
DOIs | |
Publication status | Published - 2011 |
Event | 25th Eurosensors Conference - Athens, Greece Duration: 2011 Sep 4 → 2011 Sep 7 |
Bibliographical note
Funding Information:This research was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2009-0082188, 2009-0073809, 2009-008-1529, 2010-0020767 and 2010-0020772).
All Science Journal Classification (ASJC) codes
- Engineering(all)