Abstract
Recently, we reported autodisplayed Z-domains on E.coli can highly improve the sensitivity of immunoassays through the orientation control of detection antibodies [1-3]. In this work, the amperometric analysis was applied to the immunoassay based on E.coli cells with autodisplayed Z-domains. For amperometric analaysis, the reduction current of 3,5,3′,5′-tetramethylbenzidine (TMB) was measured at the potential of -50 mV and the correlation between the reduction current and the OD value was estimated to be 28 nA/OD and the limit of detection was calculated to be 0.07 in OD unit. In this work, an immunoassay was prepared by immobilizing anti-HRP antibodies to E.coli cells with autodisplayed Z-domains and the amperometric analysis was demonstrated the quantification of bound HRP. For the demonstration of medical diagnosis, C-reactive protein (CRP) was detected by using electrochemical ELISA based on E.coli with autodisplayed Z-domains.
| Original language | English |
|---|---|
| Pages (from-to) | 944-947 |
| Number of pages | 4 |
| Journal | Procedia Engineering |
| Volume | 25 |
| DOIs | |
| Publication status | Published - 2011 Dec 1 |
| Event | 25th Eurosensors Conference - Athens, Greece Duration: 2011 Sep 4 → 2011 Sep 7 |
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All Science Journal Classification (ASJC) codes
- Engineering(all)
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Electrochemical ELISA based on E. coli with autodisplayed Z-domains. / Lee, Ju Kyung; Gu-Yoo; Min, Park; Jose, Joachim; Pyun, Jae Chul.
In: Procedia Engineering, Vol. 25, 01.12.2011, p. 944-947.Research output: Contribution to journal › Conference article
TY - JOUR
T1 - Electrochemical ELISA based on E. coli with autodisplayed Z-domains
AU - Lee, Ju Kyung
AU - Gu-Yoo,
AU - Min, Park
AU - Jose, Joachim
AU - Pyun, Jae Chul
PY - 2011/12/1
Y1 - 2011/12/1
N2 - Recently, we reported autodisplayed Z-domains on E.coli can highly improve the sensitivity of immunoassays through the orientation control of detection antibodies [1-3]. In this work, the amperometric analysis was applied to the immunoassay based on E.coli cells with autodisplayed Z-domains. For amperometric analaysis, the reduction current of 3,5,3′,5′-tetramethylbenzidine (TMB) was measured at the potential of -50 mV and the correlation between the reduction current and the OD value was estimated to be 28 nA/OD and the limit of detection was calculated to be 0.07 in OD unit. In this work, an immunoassay was prepared by immobilizing anti-HRP antibodies to E.coli cells with autodisplayed Z-domains and the amperometric analysis was demonstrated the quantification of bound HRP. For the demonstration of medical diagnosis, C-reactive protein (CRP) was detected by using electrochemical ELISA based on E.coli with autodisplayed Z-domains.
AB - Recently, we reported autodisplayed Z-domains on E.coli can highly improve the sensitivity of immunoassays through the orientation control of detection antibodies [1-3]. In this work, the amperometric analysis was applied to the immunoassay based on E.coli cells with autodisplayed Z-domains. For amperometric analaysis, the reduction current of 3,5,3′,5′-tetramethylbenzidine (TMB) was measured at the potential of -50 mV and the correlation between the reduction current and the OD value was estimated to be 28 nA/OD and the limit of detection was calculated to be 0.07 in OD unit. In this work, an immunoassay was prepared by immobilizing anti-HRP antibodies to E.coli cells with autodisplayed Z-domains and the amperometric analysis was demonstrated the quantification of bound HRP. For the demonstration of medical diagnosis, C-reactive protein (CRP) was detected by using electrochemical ELISA based on E.coli with autodisplayed Z-domains.
UR - http://www.scopus.com/inward/record.url?scp=84863178740&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863178740&partnerID=8YFLogxK
U2 - 10.1016/j.proeng.2011.12.232
DO - 10.1016/j.proeng.2011.12.232
M3 - Conference article
AN - SCOPUS:84863178740
VL - 25
SP - 944
EP - 947
JO - Procedia Engineering
JF - Procedia Engineering
SN - 1877-7058
ER -