Electrochemical ELISA based on Escherichia coli with autodisplayed Z-domains

Ju Kyung Lee, Gu-Yoo, Min Park, Joachim Jose, Min Jung Kang, Jae Chul Pyun

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Recently, we reported autodisplayed Z-domains on Escherichia coli can highly improve the sensitivity of immunoassays through the orientation control of detection antibodies. In this work, the amperometric analysis was applied to the immunoassay based on E. coli cells with autodisplayed Z-domains. For amperometric analysis, the reduction current of 3,5,3′,5′- tetramethylbenzidine (TMB) was measured at the potential of -50 mV and the correlation between the reduction current and the OD value was estimated to be 28 nA/OD and the limit of detection was calculated to be 0.07 in OD unit. In this work, the feasibility of the immunoassay based on amperometric analysis was presented by the quantification of HRP as a model analyte. For the demonstration of medical diagnosis, C-reactive protein (CRP) was detected by using electrochemical ELISA based on E. coli with autodisplayed Z-domains.

Original languageEnglish
Pages (from-to)46-52
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume175
DOIs
Publication statusPublished - 2012 Dec

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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