A diamond-like carbon (DLC) microelectrode was applied for diagnosis of RA (rheumatoid arthritis). In this work, oxidized 3,5,3',5'-tetramethylbenzidine (TMB) was quantified by amperometry instead of optical density measurement. Quantification was carried out by using a boron doped DLC microelectrode and cyclic voltammetric analysis method without quenching step which uses sulfuric acid. For the RA diagnosis, the cut-off value was determined by considering the sensitivity as well as the selectivity of the analysis. The test results demonstrate that electrochemical ELISA using DLC microelectrode was feasible for the RA diagnosis.
Bibliographical noteFunding Information:
This work was supported by KOSEF through National Core Research Center for Nanomedical Technology (No. R15-2004-024-00000-0), by the Korea Science and Engineering Foundation (KOSEF) grant founded by the Korea Goverment (MOST) (No. R01-2008-000-11767-0) and (No. 2009-0073809), and by the KIST institutional program.
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)