Electrogenerated chemiluminescence sensor based on tris(2,2'-bipyridyl) ruthenium(II) immobilized in the composite film of multi-walled carbon nanotube/sol-gel zinc oxide/Nafion

Eun Jung Choi, Chang Hoon Kang, Han Nim Choi, Wonyong Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A composite film of multi-walled carbon nanotube (MWCNT)/sol-gel-derived zinc oxide(ZnO)/Nafion has been utilized as an efficient immobilization matrix for the construction of a highly sensitive and stable tris(2,2'-bipyridyl) ruthenium(II) (Ru(bpy) 3 2+ ) electrogenerated chemiluminescence (ECL) sensor. The electrochemical and ECL behaviors of Ru(bpy) 3 2+ ion-exchanged into the composite film were strongly dependent upon the sol-gel preparation condition, the amount of MWCNT incorporated into the ZnO/Nafion composite film, and the buffer solution pH. The synergistic effect of MWCNTs and ZnO in the composite films increased not only the sensitivity but also the long-term stability of the ECL sensor. The present ECL sensor based on the MWCNT/ZnO/Nafion gave a linear response (R 2 = 0.999) for tripropylamine concentration from 500 nM to 1.0 mM with a remarkable detection limit (S/N = 3) of 15 nM. The present ECL sensor showed outstanding long-term stability (94% initial signal retained for 5 weeks). Since the present ECL sensor exhibits large response towards NADH, it could be applied as a transduction platform for the ECL biosensor in which the NADH is produced from the dehydrogenase-based enzymatic reaction in the presence of NAD + cofactor.

Original languageEnglish
Pages (from-to)2387-2392
Number of pages6
JournalBulletin of the Korean Chemical Society
Volume30
Issue number10
DOIs
Publication statusPublished - 2009 Oct 1

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Zinc Oxide
Carbon Nanotubes
Chemiluminescence
Composite films
Sol-gels
Sensors
NAD
perfluorosulfonic acid
tris(2,2'-bipyridyl)ruthenium(II)
Biosensors
Oxide films
Buffers
Oxidoreductases
Ions

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

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title = "Electrogenerated chemiluminescence sensor based on tris(2,2'-bipyridyl) ruthenium(II) immobilized in the composite film of multi-walled carbon nanotube/sol-gel zinc oxide/Nafion",
abstract = "A composite film of multi-walled carbon nanotube (MWCNT)/sol-gel-derived zinc oxide(ZnO)/Nafion has been utilized as an efficient immobilization matrix for the construction of a highly sensitive and stable tris(2,2'-bipyridyl) ruthenium(II) (Ru(bpy) 3 2+ ) electrogenerated chemiluminescence (ECL) sensor. The electrochemical and ECL behaviors of Ru(bpy) 3 2+ ion-exchanged into the composite film were strongly dependent upon the sol-gel preparation condition, the amount of MWCNT incorporated into the ZnO/Nafion composite film, and the buffer solution pH. The synergistic effect of MWCNTs and ZnO in the composite films increased not only the sensitivity but also the long-term stability of the ECL sensor. The present ECL sensor based on the MWCNT/ZnO/Nafion gave a linear response (R 2 = 0.999) for tripropylamine concentration from 500 nM to 1.0 mM with a remarkable detection limit (S/N = 3) of 15 nM. The present ECL sensor showed outstanding long-term stability (94{\%} initial signal retained for 5 weeks). Since the present ECL sensor exhibits large response towards NADH, it could be applied as a transduction platform for the ECL biosensor in which the NADH is produced from the dehydrogenase-based enzymatic reaction in the presence of NAD + cofactor.",
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AU - Lee, Wonyong

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