Electromagnetic microfluidic cell labeling device using on-chip microelectromagnet and multi-layered channels

Suk Heung Song, Hye Lyn Lee, Yoo Hong Min, Hyo il Jung

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

We present a new design for a microfluidic cell sorting system in which microelectromagnets are implemented and the Joule heating energy is dissipated to maintain a biocompatible temperature. The microfluidic channel has a multi-layered structure for rapid separation in a high gradient magnetic field. The top and bottom layers of our device both contribute to separate the magnetically labeled bio-particles, such as animal cells and bacteria, in the vertical direction. Joule heat was exploited to supply the thermal energy to an active area of cell sample channels, and the channel temperature was maintained in the biocompatible range (37 °C) using cooling channels embedded in the top channel layer. We numerically analyzed heat transfer for an on-chip electromagnet and solved the Joule heat problem of the device. Experimentally, we demonstrated the separation of a T-cell leukemia line, human Jurkat cells, utilizing immune-magnetophoresis. Magnetic beads with a characteristic polymer surface for coupling with CD3 T-cells made it possible to sort the human Jurkat cells with a labeling efficiency of greater than 95%. According to the cell viability test, the number of dead cells did not exceed 10% of the total, indicating that our cell sorting system did not cause any heat damage to the cells, despite utilizing electromagnets.

Original languageEnglish
Pages (from-to)210-216
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume141
Issue number1
DOIs
Publication statusPublished - 2009 Aug 18

Fingerprint

Microfluidics
Labeling
marking
chips
Cells
electromagnetism
Electromagnets
T-cells
cells
Sorting
electromagnets
classifying
Heat problems
heat
Joule heating
Thermal energy
Bacteria
Polymers
Animals
leukemias

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

Cite this

@article{0805b910ff824cd8ad5726d187b01ae8,
title = "Electromagnetic microfluidic cell labeling device using on-chip microelectromagnet and multi-layered channels",
abstract = "We present a new design for a microfluidic cell sorting system in which microelectromagnets are implemented and the Joule heating energy is dissipated to maintain a biocompatible temperature. The microfluidic channel has a multi-layered structure for rapid separation in a high gradient magnetic field. The top and bottom layers of our device both contribute to separate the magnetically labeled bio-particles, such as animal cells and bacteria, in the vertical direction. Joule heat was exploited to supply the thermal energy to an active area of cell sample channels, and the channel temperature was maintained in the biocompatible range (37 °C) using cooling channels embedded in the top channel layer. We numerically analyzed heat transfer for an on-chip electromagnet and solved the Joule heat problem of the device. Experimentally, we demonstrated the separation of a T-cell leukemia line, human Jurkat cells, utilizing immune-magnetophoresis. Magnetic beads with a characteristic polymer surface for coupling with CD3 T-cells made it possible to sort the human Jurkat cells with a labeling efficiency of greater than 95{\%}. According to the cell viability test, the number of dead cells did not exceed 10{\%} of the total, indicating that our cell sorting system did not cause any heat damage to the cells, despite utilizing electromagnets.",
author = "Song, {Suk Heung} and Lee, {Hye Lyn} and Min, {Yoo Hong} and Jung, {Hyo il}",
year = "2009",
month = "8",
day = "18",
doi = "10.1016/j.snb.2009.06.037",
language = "English",
volume = "141",
pages = "210--216",
journal = "Sensors and Actuators, B: Chemical",
issn = "0925-4005",
publisher = "Elsevier",
number = "1",

}

Electromagnetic microfluidic cell labeling device using on-chip microelectromagnet and multi-layered channels. / Song, Suk Heung; Lee, Hye Lyn; Min, Yoo Hong; Jung, Hyo il.

In: Sensors and Actuators, B: Chemical, Vol. 141, No. 1, 18.08.2009, p. 210-216.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Electromagnetic microfluidic cell labeling device using on-chip microelectromagnet and multi-layered channels

AU - Song, Suk Heung

AU - Lee, Hye Lyn

AU - Min, Yoo Hong

AU - Jung, Hyo il

PY - 2009/8/18

Y1 - 2009/8/18

N2 - We present a new design for a microfluidic cell sorting system in which microelectromagnets are implemented and the Joule heating energy is dissipated to maintain a biocompatible temperature. The microfluidic channel has a multi-layered structure for rapid separation in a high gradient magnetic field. The top and bottom layers of our device both contribute to separate the magnetically labeled bio-particles, such as animal cells and bacteria, in the vertical direction. Joule heat was exploited to supply the thermal energy to an active area of cell sample channels, and the channel temperature was maintained in the biocompatible range (37 °C) using cooling channels embedded in the top channel layer. We numerically analyzed heat transfer for an on-chip electromagnet and solved the Joule heat problem of the device. Experimentally, we demonstrated the separation of a T-cell leukemia line, human Jurkat cells, utilizing immune-magnetophoresis. Magnetic beads with a characteristic polymer surface for coupling with CD3 T-cells made it possible to sort the human Jurkat cells with a labeling efficiency of greater than 95%. According to the cell viability test, the number of dead cells did not exceed 10% of the total, indicating that our cell sorting system did not cause any heat damage to the cells, despite utilizing electromagnets.

AB - We present a new design for a microfluidic cell sorting system in which microelectromagnets are implemented and the Joule heating energy is dissipated to maintain a biocompatible temperature. The microfluidic channel has a multi-layered structure for rapid separation in a high gradient magnetic field. The top and bottom layers of our device both contribute to separate the magnetically labeled bio-particles, such as animal cells and bacteria, in the vertical direction. Joule heat was exploited to supply the thermal energy to an active area of cell sample channels, and the channel temperature was maintained in the biocompatible range (37 °C) using cooling channels embedded in the top channel layer. We numerically analyzed heat transfer for an on-chip electromagnet and solved the Joule heat problem of the device. Experimentally, we demonstrated the separation of a T-cell leukemia line, human Jurkat cells, utilizing immune-magnetophoresis. Magnetic beads with a characteristic polymer surface for coupling with CD3 T-cells made it possible to sort the human Jurkat cells with a labeling efficiency of greater than 95%. According to the cell viability test, the number of dead cells did not exceed 10% of the total, indicating that our cell sorting system did not cause any heat damage to the cells, despite utilizing electromagnets.

UR - http://www.scopus.com/inward/record.url?scp=67949095733&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67949095733&partnerID=8YFLogxK

U2 - 10.1016/j.snb.2009.06.037

DO - 10.1016/j.snb.2009.06.037

M3 - Article

VL - 141

SP - 210

EP - 216

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

SN - 0925-4005

IS - 1

ER -