Electrospun Silk Fibroin Nanofibrous Scaffolds with Two-Stage Hydroxyapatite Functionalization for Enhancing the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells

Eunkyung Ko, Jong Seung Lee, Hyunryung Kim, Sung Yeun Yang, Dasom Yang, Kisuk Yang, Jiyong Lee, Jisoo Shin, Hee Seok Yang, WonHyoung Ryu, Seung-Woo Cho

Research output: Contribution to journalReview article

43 Citations (Scopus)

Abstract

The development of functional scaffolds with improved osteogenic potential is important for successful bone formation and mineralization in bone tissue engineering. In this study, we developed a functional electrospun silk fibroin (SF) nanofibrous scaffold functionalized with two-stage hydroxyapatite (HAp) particles, using mussel adhesive-inspired polydopamine (PDA) chemistry. HAp particles were first incorporated into SF scaffolds during the electrospinning process, and then immobilized onto the electrospun SF nanofibrous scaffolds containing HAp via PDA-mediated adhesive chemistry. We obtained two-stage HAp-functionalized SF nanofibrous scaffolds with improved mechanical properties and capable of providing a bone-specific physiological microenvironment. The developed scaffolds were tested for their ability to enhance the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) in vitro and repair bone defect in vivo. To boost their ability for bone repair, we genetically modified hADMSCs with the transcriptional coactivator with PDZ-binding motif (TAZ) via polymer nanoparticle-mediated gene delivery. TAZ is a well-known transcriptional modulator that activates the osteogenic differentiation of mesenchymal stem cells (MSCs). Two-stage HAp-functionalized SF scaffolds significantly promoted the osteogenic differentiation of TAZ-transfected hADMSCs in vitro and enhanced mineralized bone formation in a critical-sized calvarial bone defect model. Our study shows the potential utility of SF scaffolds with nanofibrous structures and enriched inorganic components in bone tissue engineering.

Original languageEnglish
Pages (from-to)7614-7625
Number of pages12
JournalACS Applied Materials and Interfaces
Volume10
Issue number9
DOIs
Publication statusPublished - 2018 Mar 7

Fingerprint

Fibroins
Silk
Durapatite
Stem cells
Hydroxyapatite
Scaffolds
Bone
Tissue engineering
Adhesives
Repair
Defects
Electrospinning
Modulators
Polymers
Genes
Nanoparticles
Mechanical properties

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Ko, Eunkyung ; Lee, Jong Seung ; Kim, Hyunryung ; Yang, Sung Yeun ; Yang, Dasom ; Yang, Kisuk ; Lee, Jiyong ; Shin, Jisoo ; Yang, Hee Seok ; Ryu, WonHyoung ; Cho, Seung-Woo. / Electrospun Silk Fibroin Nanofibrous Scaffolds with Two-Stage Hydroxyapatite Functionalization for Enhancing the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells. In: ACS Applied Materials and Interfaces. 2018 ; Vol. 10, No. 9. pp. 7614-7625.
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Electrospun Silk Fibroin Nanofibrous Scaffolds with Two-Stage Hydroxyapatite Functionalization for Enhancing the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal Stem Cells. / Ko, Eunkyung; Lee, Jong Seung; Kim, Hyunryung; Yang, Sung Yeun; Yang, Dasom; Yang, Kisuk; Lee, Jiyong; Shin, Jisoo; Yang, Hee Seok; Ryu, WonHyoung; Cho, Seung-Woo.

In: ACS Applied Materials and Interfaces, Vol. 10, No. 9, 07.03.2018, p. 7614-7625.

Research output: Contribution to journalReview article

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AU - Ko, Eunkyung

AU - Lee, Jong Seung

AU - Kim, Hyunryung

AU - Yang, Sung Yeun

AU - Yang, Dasom

AU - Yang, Kisuk

AU - Lee, Jiyong

AU - Shin, Jisoo

AU - Yang, Hee Seok

AU - Ryu, WonHyoung

AU - Cho, Seung-Woo

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AB - The development of functional scaffolds with improved osteogenic potential is important for successful bone formation and mineralization in bone tissue engineering. In this study, we developed a functional electrospun silk fibroin (SF) nanofibrous scaffold functionalized with two-stage hydroxyapatite (HAp) particles, using mussel adhesive-inspired polydopamine (PDA) chemistry. HAp particles were first incorporated into SF scaffolds during the electrospinning process, and then immobilized onto the electrospun SF nanofibrous scaffolds containing HAp via PDA-mediated adhesive chemistry. We obtained two-stage HAp-functionalized SF nanofibrous scaffolds with improved mechanical properties and capable of providing a bone-specific physiological microenvironment. The developed scaffolds were tested for their ability to enhance the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) in vitro and repair bone defect in vivo. To boost their ability for bone repair, we genetically modified hADMSCs with the transcriptional coactivator with PDZ-binding motif (TAZ) via polymer nanoparticle-mediated gene delivery. TAZ is a well-known transcriptional modulator that activates the osteogenic differentiation of mesenchymal stem cells (MSCs). Two-stage HAp-functionalized SF scaffolds significantly promoted the osteogenic differentiation of TAZ-transfected hADMSCs in vitro and enhanced mineralized bone formation in a critical-sized calvarial bone defect model. Our study shows the potential utility of SF scaffolds with nanofibrous structures and enriched inorganic components in bone tissue engineering.

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