Elevated anti-α-enolase antibody levels in Kawasaki disease

J. K. Chun, T. J. Lee, K. M. Choi, K. H. Lee, D. S. Kim

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objective: By functioning as a heat-shock protein (HSP), α-enolase has an important role in the pathophysiology of multivariant vasculitis. Kawasaki disease (KD) is a type of vasculitis occurring primarily in children. The role of α-enolase in KD was assessed by measuring anti-α-enolase antibody (Ab) titres in patients with KD and the usefulness of anti-α-enolase Ab as a diagnostic tool in atypical KD patients was evaluated. Methods: Anti-α-enolase Ab titres were measured by using an enzyme-linked immunosorbent assay (ELISA) in seven normal control patients, nine febrile control patients and 14 KD patients (10 typical KD, four atypical KD). A standard deviation (SD) of 3 above the mean of the normal control group was considered to be positive reactivity. Western blotting using recombinant human α-enolase was performed in four KD patients and three normal controls. Results: With the positive reactivity limited to +3 SD over the mean (>0.6), 10 out of 14 patients (71%) were positive at the acute onset and 12 out of 14 patients (85.7%) were positive before discharge. In total, 12 out of 14 patients (85.7%) were positive either at acute onset or before discharge. All four atypical KD patients showed positive reactivity. Specific positive bands against recombinant human α-enolase were detected by western blotting in all four KD patients, but no reactivity was seen in three patients with normal controls. Conclusion: This is the first study to demonstrate that autoantibodies against the α-enolase are present in the sera of KD patients. We suggest that anti-α-enolase Ab should be a good candidate for a diagnostic tool in atypical KD.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalScandinavian Journal of Rheumatology
Volume37
Issue number1
DOIs
Publication statusPublished - 2008 Jan 22

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Mucocutaneous Lymph Node Syndrome
Phosphopyruvate Hydratase
Anti-Idiotypic Antibodies
Vasculitis
Western Blotting
Heat-Shock Proteins
Autoantibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Rheumatology
  • Immunology

Cite this

Chun, J. K. ; Lee, T. J. ; Choi, K. M. ; Lee, K. H. ; Kim, D. S. / Elevated anti-α-enolase antibody levels in Kawasaki disease. In: Scandinavian Journal of Rheumatology. 2008 ; Vol. 37, No. 1. pp. 48-52.
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abstract = "Objective: By functioning as a heat-shock protein (HSP), α-enolase has an important role in the pathophysiology of multivariant vasculitis. Kawasaki disease (KD) is a type of vasculitis occurring primarily in children. The role of α-enolase in KD was assessed by measuring anti-α-enolase antibody (Ab) titres in patients with KD and the usefulness of anti-α-enolase Ab as a diagnostic tool in atypical KD patients was evaluated. Methods: Anti-α-enolase Ab titres were measured by using an enzyme-linked immunosorbent assay (ELISA) in seven normal control patients, nine febrile control patients and 14 KD patients (10 typical KD, four atypical KD). A standard deviation (SD) of 3 above the mean of the normal control group was considered to be positive reactivity. Western blotting using recombinant human α-enolase was performed in four KD patients and three normal controls. Results: With the positive reactivity limited to +3 SD over the mean (>0.6), 10 out of 14 patients (71{\%}) were positive at the acute onset and 12 out of 14 patients (85.7{\%}) were positive before discharge. In total, 12 out of 14 patients (85.7{\%}) were positive either at acute onset or before discharge. All four atypical KD patients showed positive reactivity. Specific positive bands against recombinant human α-enolase were detected by western blotting in all four KD patients, but no reactivity was seen in three patients with normal controls. Conclusion: This is the first study to demonstrate that autoantibodies against the α-enolase are present in the sera of KD patients. We suggest that anti-α-enolase Ab should be a good candidate for a diagnostic tool in atypical KD.",
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Elevated anti-α-enolase antibody levels in Kawasaki disease. / Chun, J. K.; Lee, T. J.; Choi, K. M.; Lee, K. H.; Kim, D. S.

In: Scandinavian Journal of Rheumatology, Vol. 37, No. 1, 22.01.2008, p. 48-52.

Research output: Contribution to journalArticle

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T1 - Elevated anti-α-enolase antibody levels in Kawasaki disease

AU - Chun, J. K.

AU - Lee, T. J.

AU - Choi, K. M.

AU - Lee, K. H.

AU - Kim, D. S.

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N2 - Objective: By functioning as a heat-shock protein (HSP), α-enolase has an important role in the pathophysiology of multivariant vasculitis. Kawasaki disease (KD) is a type of vasculitis occurring primarily in children. The role of α-enolase in KD was assessed by measuring anti-α-enolase antibody (Ab) titres in patients with KD and the usefulness of anti-α-enolase Ab as a diagnostic tool in atypical KD patients was evaluated. Methods: Anti-α-enolase Ab titres were measured by using an enzyme-linked immunosorbent assay (ELISA) in seven normal control patients, nine febrile control patients and 14 KD patients (10 typical KD, four atypical KD). A standard deviation (SD) of 3 above the mean of the normal control group was considered to be positive reactivity. Western blotting using recombinant human α-enolase was performed in four KD patients and three normal controls. Results: With the positive reactivity limited to +3 SD over the mean (>0.6), 10 out of 14 patients (71%) were positive at the acute onset and 12 out of 14 patients (85.7%) were positive before discharge. In total, 12 out of 14 patients (85.7%) were positive either at acute onset or before discharge. All four atypical KD patients showed positive reactivity. Specific positive bands against recombinant human α-enolase were detected by western blotting in all four KD patients, but no reactivity was seen in three patients with normal controls. Conclusion: This is the first study to demonstrate that autoantibodies against the α-enolase are present in the sera of KD patients. We suggest that anti-α-enolase Ab should be a good candidate for a diagnostic tool in atypical KD.

AB - Objective: By functioning as a heat-shock protein (HSP), α-enolase has an important role in the pathophysiology of multivariant vasculitis. Kawasaki disease (KD) is a type of vasculitis occurring primarily in children. The role of α-enolase in KD was assessed by measuring anti-α-enolase antibody (Ab) titres in patients with KD and the usefulness of anti-α-enolase Ab as a diagnostic tool in atypical KD patients was evaluated. Methods: Anti-α-enolase Ab titres were measured by using an enzyme-linked immunosorbent assay (ELISA) in seven normal control patients, nine febrile control patients and 14 KD patients (10 typical KD, four atypical KD). A standard deviation (SD) of 3 above the mean of the normal control group was considered to be positive reactivity. Western blotting using recombinant human α-enolase was performed in four KD patients and three normal controls. Results: With the positive reactivity limited to +3 SD over the mean (>0.6), 10 out of 14 patients (71%) were positive at the acute onset and 12 out of 14 patients (85.7%) were positive before discharge. In total, 12 out of 14 patients (85.7%) were positive either at acute onset or before discharge. All four atypical KD patients showed positive reactivity. Specific positive bands against recombinant human α-enolase were detected by western blotting in all four KD patients, but no reactivity was seen in three patients with normal controls. Conclusion: This is the first study to demonstrate that autoantibodies against the α-enolase are present in the sera of KD patients. We suggest that anti-α-enolase Ab should be a good candidate for a diagnostic tool in atypical KD.

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