Elevated interleukin-32 expression in granulomatosis with polyangiitis

Suyoung Bae, Yong Gil Kim, Jida Choi, Jaewoo Hong, Siyoung Lee, Taebong Kang, Hyunjhung Jeon, Kwangwon Hong, Eusom Kim, Areum Kwak, Chang Keun Lee, Bin Yoo, YongBeom Park, Eun Young Song, Soohyun Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: To evaluate the role of IL-32 in granulomatosis with polyangiitis (GPA) patients and the relationship between IL-32 and disease activity, as PR3 has the ability to bind and activate IL-32, which has been described as a novel cytokine that induces inflammatory cytokines. Methods: We investigated the level of IL-32, PR3, TNF-α and IL-6 in GPA patients by using ELISA. Northern blot was used to analyse the level of IL-32 mRNA in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes was examined by IF staining. Results: We observed that IL-32 and PR3 levels in GPA patients were increased significantly when compared with normal individuals and each was tightly associated (P < 0.001). Northern blot analysis revealed that the mRNA level of IL-32 was prominently elevated in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes from GPA patients vs normal individuals was verified by IF staining. Conclusion: IL-32 level was elevated in GPA patients but its level was changed by treatment response. IL-32 could be an index in GPA and play a role in the aetiology of GPA.

Original languageEnglish
Article numberkes163
Pages (from-to)1979-1988
Number of pages10
JournalRheumatology (United Kingdom)
Volume51
Issue number11
DOIs
Publication statusPublished - 2012 Nov 1

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Granulomatosis with Polyangiitis
Interleukins
Leukocytes
Northern Blotting
Staining and Labeling
Cytokines
Messenger RNA
Interleukin-6
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Pharmacology (medical)

Cite this

Bae, S., Kim, Y. G., Choi, J., Hong, J., Lee, S., Kang, T., ... Kim, S. (2012). Elevated interleukin-32 expression in granulomatosis with polyangiitis. Rheumatology (United Kingdom), 51(11), 1979-1988. [kes163]. https://doi.org/10.1093/rheumatology/kes163
Bae, Suyoung ; Kim, Yong Gil ; Choi, Jida ; Hong, Jaewoo ; Lee, Siyoung ; Kang, Taebong ; Jeon, Hyunjhung ; Hong, Kwangwon ; Kim, Eusom ; Kwak, Areum ; Lee, Chang Keun ; Yoo, Bin ; Park, YongBeom ; Song, Eun Young ; Kim, Soohyun. / Elevated interleukin-32 expression in granulomatosis with polyangiitis. In: Rheumatology (United Kingdom). 2012 ; Vol. 51, No. 11. pp. 1979-1988.
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title = "Elevated interleukin-32 expression in granulomatosis with polyangiitis",
abstract = "Objective: To evaluate the role of IL-32 in granulomatosis with polyangiitis (GPA) patients and the relationship between IL-32 and disease activity, as PR3 has the ability to bind and activate IL-32, which has been described as a novel cytokine that induces inflammatory cytokines. Methods: We investigated the level of IL-32, PR3, TNF-α and IL-6 in GPA patients by using ELISA. Northern blot was used to analyse the level of IL-32 mRNA in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes was examined by IF staining. Results: We observed that IL-32 and PR3 levels in GPA patients were increased significantly when compared with normal individuals and each was tightly associated (P < 0.001). Northern blot analysis revealed that the mRNA level of IL-32 was prominently elevated in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes from GPA patients vs normal individuals was verified by IF staining. Conclusion: IL-32 level was elevated in GPA patients but its level was changed by treatment response. IL-32 could be an index in GPA and play a role in the aetiology of GPA.",
author = "Suyoung Bae and Kim, {Yong Gil} and Jida Choi and Jaewoo Hong and Siyoung Lee and Taebong Kang and Hyunjhung Jeon and Kwangwon Hong and Eusom Kim and Areum Kwak and Lee, {Chang Keun} and Bin Yoo and YongBeom Park and Song, {Eun Young} and Soohyun Kim",
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Bae, S, Kim, YG, Choi, J, Hong, J, Lee, S, Kang, T, Jeon, H, Hong, K, Kim, E, Kwak, A, Lee, CK, Yoo, B, Park, Y, Song, EY & Kim, S 2012, 'Elevated interleukin-32 expression in granulomatosis with polyangiitis', Rheumatology (United Kingdom), vol. 51, no. 11, kes163, pp. 1979-1988. https://doi.org/10.1093/rheumatology/kes163

Elevated interleukin-32 expression in granulomatosis with polyangiitis. / Bae, Suyoung; Kim, Yong Gil; Choi, Jida; Hong, Jaewoo; Lee, Siyoung; Kang, Taebong; Jeon, Hyunjhung; Hong, Kwangwon; Kim, Eusom; Kwak, Areum; Lee, Chang Keun; Yoo, Bin; Park, YongBeom; Song, Eun Young; Kim, Soohyun.

In: Rheumatology (United Kingdom), Vol. 51, No. 11, kes163, 01.11.2012, p. 1979-1988.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Elevated interleukin-32 expression in granulomatosis with polyangiitis

AU - Bae, Suyoung

AU - Kim, Yong Gil

AU - Choi, Jida

AU - Hong, Jaewoo

AU - Lee, Siyoung

AU - Kang, Taebong

AU - Jeon, Hyunjhung

AU - Hong, Kwangwon

AU - Kim, Eusom

AU - Kwak, Areum

AU - Lee, Chang Keun

AU - Yoo, Bin

AU - Park, YongBeom

AU - Song, Eun Young

AU - Kim, Soohyun

PY - 2012/11/1

Y1 - 2012/11/1

N2 - Objective: To evaluate the role of IL-32 in granulomatosis with polyangiitis (GPA) patients and the relationship between IL-32 and disease activity, as PR3 has the ability to bind and activate IL-32, which has been described as a novel cytokine that induces inflammatory cytokines. Methods: We investigated the level of IL-32, PR3, TNF-α and IL-6 in GPA patients by using ELISA. Northern blot was used to analyse the level of IL-32 mRNA in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes was examined by IF staining. Results: We observed that IL-32 and PR3 levels in GPA patients were increased significantly when compared with normal individuals and each was tightly associated (P < 0.001). Northern blot analysis revealed that the mRNA level of IL-32 was prominently elevated in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes from GPA patients vs normal individuals was verified by IF staining. Conclusion: IL-32 level was elevated in GPA patients but its level was changed by treatment response. IL-32 could be an index in GPA and play a role in the aetiology of GPA.

AB - Objective: To evaluate the role of IL-32 in granulomatosis with polyangiitis (GPA) patients and the relationship between IL-32 and disease activity, as PR3 has the ability to bind and activate IL-32, which has been described as a novel cytokine that induces inflammatory cytokines. Methods: We investigated the level of IL-32, PR3, TNF-α and IL-6 in GPA patients by using ELISA. Northern blot was used to analyse the level of IL-32 mRNA in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes was examined by IF staining. Results: We observed that IL-32 and PR3 levels in GPA patients were increased significantly when compared with normal individuals and each was tightly associated (P < 0.001). Northern blot analysis revealed that the mRNA level of IL-32 was prominently elevated in leucocytes of GPA patients. The intracellular colocalization of IL-32 and PR3 in leucocytes from GPA patients vs normal individuals was verified by IF staining. Conclusion: IL-32 level was elevated in GPA patients but its level was changed by treatment response. IL-32 could be an index in GPA and play a role in the aetiology of GPA.

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