Elevated level of human RPA interacting protein α (hRIPα) in cervical tumor cells is involved in cell proliferation through regulating RPA transport

Sim Namkoong, Eun Ju Lee, Ik Soon Jang, Junsoo Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Replication protein A (RPA) is a eukaryotic single-stranded DNA binding protein that is essential for DNA replication, repair, and recombination, and human RPA interacting protein α (hRIPα) is the nuclear transporter of RPA. Here, we report the regulatory role of hRIPα protein in cell proliferation. Western blot analysis revealed that the level of hRIPα was frequently elevated in cervical tumors tissues and hRIPα knockdown by siRNA inhibited cellular proliferation through deregulation of the cell cycle. In addition, overexpression of hRIPα resulted in increased clonogenicity. These results indicate that hRIPα is involved in cell proliferation through regulation of RPA transport.

Original languageEnglish
Pages (from-to)3753-3760
Number of pages8
JournalFEBS Letters
Volume586
Issue number20
DOIs
Publication statusPublished - 2012 Oct 19

Fingerprint

Replication Protein A
Cell proliferation
Protein Transport
Tumors
Cells
Cell Proliferation
Neoplasms
Proteins
DNA-Binding Proteins
Nuclear Proteins
Deregulation
DNA Replication
DNA Repair
Small Interfering RNA
Genetic Recombination
human RPA1 protein
Cell Cycle
Western Blotting
Repair
Tissue

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

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abstract = "Replication protein A (RPA) is a eukaryotic single-stranded DNA binding protein that is essential for DNA replication, repair, and recombination, and human RPA interacting protein α (hRIPα) is the nuclear transporter of RPA. Here, we report the regulatory role of hRIPα protein in cell proliferation. Western blot analysis revealed that the level of hRIPα was frequently elevated in cervical tumors tissues and hRIPα knockdown by siRNA inhibited cellular proliferation through deregulation of the cell cycle. In addition, overexpression of hRIPα resulted in increased clonogenicity. These results indicate that hRIPα is involved in cell proliferation through regulation of RPA transport.",
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Elevated level of human RPA interacting protein α (hRIPα) in cervical tumor cells is involved in cell proliferation through regulating RPA transport. / Namkoong, Sim; Lee, Eun Ju; Jang, Ik Soon; Park, Junsoo.

In: FEBS Letters, Vol. 586, No. 20, 19.10.2012, p. 3753-3760.

Research output: Contribution to journalArticle

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