ELISA inhibition test using monoclonal antibody specific for Treponema pallidum as the serologies test for syphilis

K. W. Whang, M. G. Lee, M. S. Song, J. B. Lee

Research output: Contribution to journalArticle

Abstract

Monoclonal antibodies (Mabs) specific for Treponema pallidum were produced using hybridoma technology. In this study an ELISA inhibition test based on competitive inhibition by antibodies from human test sera of the binding of the enzyme-labelled Mabs to T. pallidum was performed. Inhibition of the seroreactivity of Mabs was decreased according to the dilution of rabbit antiserum to T. pallidum. Seropositivity was found in 100% of secondary and early latent syphilis patients, 75% of primary syphilis patients, and 80% of late latent syphilis patients. The mean percentage inhibition was significantly higher in each syphilis group than in the controls, and statistically significant differences were shown between all the syphilis groups. VDRL and TPHA titers were correlated with the percentage inhibitions. Therefore the ELISA inhibition test using Mab specific for T. pallidum might well be a suitable tool as a new serologic test for syphilis.

Original languageEnglish
Pages (from-to)397-399
Number of pages3
JournalActa Dermato-Venereologica
Volume75
Issue number5
Publication statusPublished - 1995 Jan 1

Fingerprint

Treponema pallidum
Serology
Syphilis
Latent Syphilis
Enzyme-Linked Immunosorbent Assay
Monoclonal Antibodies
Hybridomas
Serologic Tests
Immune Sera
Rabbits
Technology
Control Groups
Antibodies
Enzymes
Serum

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

@article{68e7d32be042414a87fdbe0e4014fdc1,
title = "ELISA inhibition test using monoclonal antibody specific for Treponema pallidum as the serologies test for syphilis",
abstract = "Monoclonal antibodies (Mabs) specific for Treponema pallidum were produced using hybridoma technology. In this study an ELISA inhibition test based on competitive inhibition by antibodies from human test sera of the binding of the enzyme-labelled Mabs to T. pallidum was performed. Inhibition of the seroreactivity of Mabs was decreased according to the dilution of rabbit antiserum to T. pallidum. Seropositivity was found in 100{\%} of secondary and early latent syphilis patients, 75{\%} of primary syphilis patients, and 80{\%} of late latent syphilis patients. The mean percentage inhibition was significantly higher in each syphilis group than in the controls, and statistically significant differences were shown between all the syphilis groups. VDRL and TPHA titers were correlated with the percentage inhibitions. Therefore the ELISA inhibition test using Mab specific for T. pallidum might well be a suitable tool as a new serologic test for syphilis.",
author = "Whang, {K. W.} and Lee, {M. G.} and Song, {M. S.} and Lee, {J. B.}",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "75",
pages = "397--399",
journal = "Acta Dermato-Venereologica",
issn = "0001-5555",
publisher = "Society for the Publication of Acta Dermato-Venereologica",
number = "5",

}

ELISA inhibition test using monoclonal antibody specific for Treponema pallidum as the serologies test for syphilis. / Whang, K. W.; Lee, M. G.; Song, M. S.; Lee, J. B.

In: Acta Dermato-Venereologica, Vol. 75, No. 5, 01.01.1995, p. 397-399.

Research output: Contribution to journalArticle

TY - JOUR

T1 - ELISA inhibition test using monoclonal antibody specific for Treponema pallidum as the serologies test for syphilis

AU - Whang, K. W.

AU - Lee, M. G.

AU - Song, M. S.

AU - Lee, J. B.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Monoclonal antibodies (Mabs) specific for Treponema pallidum were produced using hybridoma technology. In this study an ELISA inhibition test based on competitive inhibition by antibodies from human test sera of the binding of the enzyme-labelled Mabs to T. pallidum was performed. Inhibition of the seroreactivity of Mabs was decreased according to the dilution of rabbit antiserum to T. pallidum. Seropositivity was found in 100% of secondary and early latent syphilis patients, 75% of primary syphilis patients, and 80% of late latent syphilis patients. The mean percentage inhibition was significantly higher in each syphilis group than in the controls, and statistically significant differences were shown between all the syphilis groups. VDRL and TPHA titers were correlated with the percentage inhibitions. Therefore the ELISA inhibition test using Mab specific for T. pallidum might well be a suitable tool as a new serologic test for syphilis.

AB - Monoclonal antibodies (Mabs) specific for Treponema pallidum were produced using hybridoma technology. In this study an ELISA inhibition test based on competitive inhibition by antibodies from human test sera of the binding of the enzyme-labelled Mabs to T. pallidum was performed. Inhibition of the seroreactivity of Mabs was decreased according to the dilution of rabbit antiserum to T. pallidum. Seropositivity was found in 100% of secondary and early latent syphilis patients, 75% of primary syphilis patients, and 80% of late latent syphilis patients. The mean percentage inhibition was significantly higher in each syphilis group than in the controls, and statistically significant differences were shown between all the syphilis groups. VDRL and TPHA titers were correlated with the percentage inhibitions. Therefore the ELISA inhibition test using Mab specific for T. pallidum might well be a suitable tool as a new serologic test for syphilis.

UR - http://www.scopus.com/inward/record.url?scp=0028784571&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028784571&partnerID=8YFLogxK

M3 - Article

C2 - 8615063

AN - SCOPUS:0028784571

VL - 75

SP - 397

EP - 399

JO - Acta Dermato-Venereologica

JF - Acta Dermato-Venereologica

SN - 0001-5555

IS - 5

ER -