Emergence of CTX-M-12 extended-spectrum β-lactamase-producing Escherichia coli in Korea

Il Kwon Bae, You Nae Lee, Hyun Yong Hwang, Seokhoon Jeong, Su Jin Lee, Hyo Sun Kwak, Wonkeun Song, Hyoung Jin Kim, Hasik Youn

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objectives: To characterize CTX-M-12 extended-spectrum β-lactamase (ESBL) produced by clinical Escherichia coli isolates and to investigate its genetic environment. Methods: Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the double-disc synergy test was carried out. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the genetic environments of the bla CTX-M-12 genes were investigated by PCR and sequencing of the regions surrounding the genes. Kinetic parameters were determined from purified CTX-M-12. Results: Sequence data for the CTX-M-1 cluster from three clinical E. coli isolates indicated the presence of CTX-M-12. An IS Ecp1 insertion sequence was located 49 bp upstream of bla CTX-M-12 in all three E. coli isolates. CTX-M-12 had a more potent hydrolytic activity against cefotaxime than against ceftazidime and was encoded on a self-transferable ∼18 kbp plasmid. Conclusions: This work shows that CTX-M-12, which confers high-level resistance to cefotaxime but not to ceftazidime, has emerged in Korea. The blaCTX-M-12 gene was associated with an upstream IS Ecp1 insertion sequence.

Original languageEnglish
Pages (from-to)1257-1259
Number of pages3
JournalJournal of Antimicrobial Chemotherapy
Volume58
Issue number6
DOIs
Publication statusPublished - 2006 Dec 1

Fingerprint

Korea
Escherichia coli
Ceftazidime
Cefotaxime
Insertional Mutagenesis
Genes
Polymerase Chain Reaction
beta-lactamase CTX-2
Agar
Plasmids

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Bae, Il Kwon ; Lee, You Nae ; Hwang, Hyun Yong ; Jeong, Seokhoon ; Lee, Su Jin ; Kwak, Hyo Sun ; Song, Wonkeun ; Kim, Hyoung Jin ; Youn, Hasik. / Emergence of CTX-M-12 extended-spectrum β-lactamase-producing Escherichia coli in Korea. In: Journal of Antimicrobial Chemotherapy. 2006 ; Vol. 58, No. 6. pp. 1257-1259.
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abstract = "Objectives: To characterize CTX-M-12 extended-spectrum β-lactamase (ESBL) produced by clinical Escherichia coli isolates and to investigate its genetic environment. Methods: Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the double-disc synergy test was carried out. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the genetic environments of the bla CTX-M-12 genes were investigated by PCR and sequencing of the regions surrounding the genes. Kinetic parameters were determined from purified CTX-M-12. Results: Sequence data for the CTX-M-1 cluster from three clinical E. coli isolates indicated the presence of CTX-M-12. An IS Ecp1 insertion sequence was located 49 bp upstream of bla CTX-M-12 in all three E. coli isolates. CTX-M-12 had a more potent hydrolytic activity against cefotaxime than against ceftazidime and was encoded on a self-transferable ∼18 kbp plasmid. Conclusions: This work shows that CTX-M-12, which confers high-level resistance to cefotaxime but not to ceftazidime, has emerged in Korea. The blaCTX-M-12 gene was associated with an upstream IS Ecp1 insertion sequence.",
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Bae, IK, Lee, YN, Hwang, HY, Jeong, S, Lee, SJ, Kwak, HS, Song, W, Kim, HJ & Youn, H 2006, 'Emergence of CTX-M-12 extended-spectrum β-lactamase-producing Escherichia coli in Korea', Journal of Antimicrobial Chemotherapy, vol. 58, no. 6, pp. 1257-1259. https://doi.org/10.1093/jac/dkl397

Emergence of CTX-M-12 extended-spectrum β-lactamase-producing Escherichia coli in Korea. / Bae, Il Kwon; Lee, You Nae; Hwang, Hyun Yong; Jeong, Seokhoon; Lee, Su Jin; Kwak, Hyo Sun; Song, Wonkeun; Kim, Hyoung Jin; Youn, Hasik.

In: Journal of Antimicrobial Chemotherapy, Vol. 58, No. 6, 01.12.2006, p. 1257-1259.

Research output: Contribution to journalArticle

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AU - Bae, Il Kwon

AU - Lee, You Nae

AU - Hwang, Hyun Yong

AU - Jeong, Seokhoon

AU - Lee, Su Jin

AU - Kwak, Hyo Sun

AU - Song, Wonkeun

AU - Kim, Hyoung Jin

AU - Youn, Hasik

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N2 - Objectives: To characterize CTX-M-12 extended-spectrum β-lactamase (ESBL) produced by clinical Escherichia coli isolates and to investigate its genetic environment. Methods: Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the double-disc synergy test was carried out. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the genetic environments of the bla CTX-M-12 genes were investigated by PCR and sequencing of the regions surrounding the genes. Kinetic parameters were determined from purified CTX-M-12. Results: Sequence data for the CTX-M-1 cluster from three clinical E. coli isolates indicated the presence of CTX-M-12. An IS Ecp1 insertion sequence was located 49 bp upstream of bla CTX-M-12 in all three E. coli isolates. CTX-M-12 had a more potent hydrolytic activity against cefotaxime than against ceftazidime and was encoded on a self-transferable ∼18 kbp plasmid. Conclusions: This work shows that CTX-M-12, which confers high-level resistance to cefotaxime but not to ceftazidime, has emerged in Korea. The blaCTX-M-12 gene was associated with an upstream IS Ecp1 insertion sequence.

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