Abstract
Objectives: To characterize CTX-M-12 extended-spectrum β-lactamase (ESBL) produced by clinical Escherichia coli isolates and to investigate its genetic environment. Methods: Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the double-disc synergy test was carried out. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the genetic environments of the bla CTX-M-12 genes were investigated by PCR and sequencing of the regions surrounding the genes. Kinetic parameters were determined from purified CTX-M-12. Results: Sequence data for the CTX-M-1 cluster from three clinical E. coli isolates indicated the presence of CTX-M-12. An IS Ecp1 insertion sequence was located 49 bp upstream of bla CTX-M-12 in all three E. coli isolates. CTX-M-12 had a more potent hydrolytic activity against cefotaxime than against ceftazidime and was encoded on a self-transferable ∼18 kbp plasmid. Conclusions: This work shows that CTX-M-12, which confers high-level resistance to cefotaxime but not to ceftazidime, has emerged in Korea. The blaCTX-M-12 gene was associated with an upstream IS Ecp1 insertion sequence.
| Original language | English |
|---|---|
| Pages (from-to) | 1257-1259 |
| Number of pages | 3 |
| Journal | Journal of Antimicrobial Chemotherapy |
| Volume | 58 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2006 Dec 1 |
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All Science Journal Classification (ASJC) codes
- Pharmacology
- Pharmacology (medical)
- Infectious Diseases
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}
Emergence of CTX-M-12 extended-spectrum β-lactamase-producing Escherichia coli in Korea. / Bae, Il Kwon; Lee, You Nae; Hwang, Hyun Yong; Jeong, Seokhoon; Lee, Su Jin; Kwak, Hyo Sun; Song, Wonkeun; Kim, Hyoung Jin; Youn, Hasik.
In: Journal of Antimicrobial Chemotherapy, Vol. 58, No. 6, 01.12.2006, p. 1257-1259.Research output: Contribution to journal › Article
TY - JOUR
T1 - Emergence of CTX-M-12 extended-spectrum β-lactamase-producing Escherichia coli in Korea
AU - Bae, Il Kwon
AU - Lee, You Nae
AU - Hwang, Hyun Yong
AU - Jeong, Seokhoon
AU - Lee, Su Jin
AU - Kwak, Hyo Sun
AU - Song, Wonkeun
AU - Kim, Hyoung Jin
AU - Youn, Hasik
PY - 2006/12/1
Y1 - 2006/12/1
N2 - Objectives: To characterize CTX-M-12 extended-spectrum β-lactamase (ESBL) produced by clinical Escherichia coli isolates and to investigate its genetic environment. Methods: Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the double-disc synergy test was carried out. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the genetic environments of the bla CTX-M-12 genes were investigated by PCR and sequencing of the regions surrounding the genes. Kinetic parameters were determined from purified CTX-M-12. Results: Sequence data for the CTX-M-1 cluster from three clinical E. coli isolates indicated the presence of CTX-M-12. An IS Ecp1 insertion sequence was located 49 bp upstream of bla CTX-M-12 in all three E. coli isolates. CTX-M-12 had a more potent hydrolytic activity against cefotaxime than against ceftazidime and was encoded on a self-transferable ∼18 kbp plasmid. Conclusions: This work shows that CTX-M-12, which confers high-level resistance to cefotaxime but not to ceftazidime, has emerged in Korea. The blaCTX-M-12 gene was associated with an upstream IS Ecp1 insertion sequence.
AB - Objectives: To characterize CTX-M-12 extended-spectrum β-lactamase (ESBL) produced by clinical Escherichia coli isolates and to investigate its genetic environment. Methods: Antimicrobial susceptibilities were determined by disc diffusion and agar dilution methods, and the double-disc synergy test was carried out. Detection of genes encoding class A β-lactamases was performed by PCR amplification, and the genetic environments of the bla CTX-M-12 genes were investigated by PCR and sequencing of the regions surrounding the genes. Kinetic parameters were determined from purified CTX-M-12. Results: Sequence data for the CTX-M-1 cluster from three clinical E. coli isolates indicated the presence of CTX-M-12. An IS Ecp1 insertion sequence was located 49 bp upstream of bla CTX-M-12 in all three E. coli isolates. CTX-M-12 had a more potent hydrolytic activity against cefotaxime than against ceftazidime and was encoded on a self-transferable ∼18 kbp plasmid. Conclusions: This work shows that CTX-M-12, which confers high-level resistance to cefotaxime but not to ceftazidime, has emerged in Korea. The blaCTX-M-12 gene was associated with an upstream IS Ecp1 insertion sequence.
UR - http://www.scopus.com/inward/record.url?scp=33845369128&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33845369128&partnerID=8YFLogxK
U2 - 10.1093/jac/dkl397
DO - 10.1093/jac/dkl397
M3 - Article
C2 - 17003059
AN - SCOPUS:33845369128
VL - 58
SP - 1257
EP - 1259
JO - Journal of Antimicrobial Chemotherapy
JF - Journal of Antimicrobial Chemotherapy
SN - 0305-7453
IS - 6
ER -