Emergence of multidrug-resistant Salmonella enterica serovar Typhi in Korea

Kyungwon Lee, DongEun Yong, Jong Hwa Yum, Young Sik Lim, Hyonsuk Kim, Bok Kwon Lee, Yunsop Chong

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A chloramphenicol-resistant strain of Salmonella enterica serovar Typhi was first noted in Korea in 1992, when a resistant isolate was detected in a returned traveler. Continued isolation of multidrug-resistant (MDR) strains thereafter in other settings prompted a retrospective analysis of laboratory records and phenotypic and genotypic analyses of 12 chloramphenicol-resistant isolates. Among these, one isolate was resistant only to chloramphenicol, and the other isolates were also resistant to ampicillin and co-trimoxazole. MDR was transferred by conjugation from 9 of the 11 isolates. PCR showed that all isolates had an incompatible group HI1 plasmid, and oriT was detected in 10 isolates, which included strains with an unsuccessful transfer of resistance. All of the ampicillin-resistant isolates had a β-lactamase band of pI 5.4 and blaTEM alleles. A PCR amplicon from an isolate showed that the sequences were identical to those of blaTEM-1, suggesting that all isolates had a TEM-1 β-lactamase. All isolates had class 1 integrons: 10 isolates had integrons of ca. 1.2 kb with dhfr7 gene cassettes, and 1 isolate had an integron of ca. 2.3 kb with aacA4 and blaOXA-1-like gene cassettes. The pulsed-field gel electrophoresis patterns of 7 of 11 MDR isolates were identical and indistinguishable from those reported for isolates in India and Indonesia. In conclusion, some of the MDR strains in Korea are related to those in other Asian countries. Susceptibility testing became necessary for selection of antimicrobial agents for the optimal treatment of patients with the emergence of MDR Salmonella serovar Typhi in Korea.

Original languageEnglish
Pages (from-to)4130-4135
Number of pages6
JournalAntimicrobial agents and chemotherapy
Volume48
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1

Fingerprint

Integrons
Salmonella typhi
Chloramphenicol
Korea
Ampicillin
Polymerase Chain Reaction
Indonesia
Pulsed Field Gel Electrophoresis
Sulfamethoxazole Drug Combination Trimethoprim
Anti-Infective Agents
Genes
India
Plasmids
Alleles
Therapeutics

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Lee, Kyungwon ; Yong, DongEun ; Yum, Jong Hwa ; Lim, Young Sik ; Kim, Hyonsuk ; Lee, Bok Kwon ; Chong, Yunsop. / Emergence of multidrug-resistant Salmonella enterica serovar Typhi in Korea. In: Antimicrobial agents and chemotherapy. 2004 ; Vol. 48, No. 11. pp. 4130-4135.
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Emergence of multidrug-resistant Salmonella enterica serovar Typhi in Korea. / Lee, Kyungwon; Yong, DongEun; Yum, Jong Hwa; Lim, Young Sik; Kim, Hyonsuk; Lee, Bok Kwon; Chong, Yunsop.

In: Antimicrobial agents and chemotherapy, Vol. 48, No. 11, 01.11.2004, p. 4130-4135.

Research output: Contribution to journalArticle

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AU - Yum, Jong Hwa

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