Encapsulated magnetic nanoparticles as supports for proteins and recyclable biocatalysts

Aimee R. Herdt, Byeong-Su Kim, T. Andrew Taton

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

This paper describes the bioconjugation of histidine-tagged enzymes and other proteins to the surface of composite "magnetomicelles" consisting of magnetic γ-Fe2O3 nanoparticles encapsulated within cross-linked polystyrene-block-polyacrylate copolymer micelle shells. Free carboxylic acid groups on the magnetomicelle surface were converted to Cu2+-iminodiacetic acid (IDA) for protein capture. The conjugation of T4 DNA ligase and enhanced green fluorescent protein to magnetomicelles revealed that proteins were captured with a high surface density and could be magnetically separated from reaction mixtures and subsequently released from the nanoparticle surface. Additionally, bioconjugation of T7 RNA polymerase yielded a functional enzyme that maintained its biological activity and could be recycled for up to three subsequent transcription reactions. We propose that protein-magnetomicelle bioconjugates are effective for protein bioseparation and enzymatic recycling and further strengthen the idea that nanoparticle surfaces have utility in protein immobilization.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalBioconjugate Chemistry
Volume18
Issue number1
DOIs
Publication statusPublished - 2007 Jan 1

Fingerprint

Biocatalysts
Catalyst supports
Nanoparticles
Proteins
Enzymes
DNA Ligases
Polystyrenes
Micelles
Carboxylic Acids
Histidine
Immobilization
Transcription
Bioactivity
Polyacrylates
Membrane Proteins
Recycling
RNA
Carboxylic acids
Copolymers
DNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

Cite this

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Encapsulated magnetic nanoparticles as supports for proteins and recyclable biocatalysts. / Herdt, Aimee R.; Kim, Byeong-Su; Taton, T. Andrew.

In: Bioconjugate Chemistry, Vol. 18, No. 1, 01.01.2007, p. 183-189.

Research output: Contribution to journalArticle

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