Encapsulation of mouse stem cells in alginate bead by microfluidic chip

Su Kyoung Chae, In Hye Lee, Choong Kim, Chang Ha Lee, Ji Yoon Kang, Tae Song Kim

Research output: Contribution to conferencePaper

1 Citation (Scopus)

Abstract

A highly efficient droplet-generating microfluidic chip for encapsulating cells with hydrogel is presented. The chip has channels of several tens of micrometers for extruding alginate aqueous solution containing the P19 embryonic carcinoma stem cells and immiscible mineral oil. The cross junction of two heterogeneous flows was able to generate alginate droplets based on Rayleigh-Plateau instability. The electrostatic stabilized alginate solution was prepared by mixing 0.05 % poly-L-lysine and 1 % alginate solution. The diameter and the frequency of alginate droplets were controlled by the concentration of alginate solution and the velocity of running fluids. The alginate droplets in oil flowed into reservoir of calcium ion solution and reacted with ions instantaneously to form hydrogel bead. As a result, cells were quickly encapsulated in synthesized alginate hydrogel beads of 100∼200 μm in diameter in microfluidic chip. These encapsulation beads were applicable to the encapsulation of single or several stem cells leading to the embryonic body formation for stem cell differentiation.

Original languageEnglish
Pages1471-1473
Number of pages3
Publication statusPublished - 2006 Jan 1
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period06/11/506/11/9

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Chae, S. K., Lee, I. H., Kim, C., Lee, C. H., Kang, J. Y., & Kim, T. S. (2006). Encapsulation of mouse stem cells in alginate bead by microfluidic chip. 1471-1473. Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.