Endostatin binds to the catalytic domain of matrix metalloproteinase-2

Seo Jin Lee, Jin Wook Jang, Young Mi Kim, Hyun Ick Lee, Jun Yung Jeon, Young Guen Kwon, Seung Taek Lee

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

We previously reported that endostatin inhibits endothelial and tumor cellular invasion by blocking activation and catalytic activity of matrix metalloproteinase (MMP)-2. Here we have examined the domain of proMMP-2 responsible for the binding of endostatin using surface plasmon resonance. ProMMP-2 and proMMP-2ΔHP lacking the hinge and hemopexin-like (HP) domains bound little to the immobilized endostatin. The active MMP-2 and MMP-2ΔHP, but not the HP domain of MMP-2, bound to endostatin at similar levels. In addition, preincubation of MMP-2 and MMP-2ΔHP with the MMP inhibitor actinonin, which binds to the active site of MMP-2, abolished their bindings to endostatin. These results indicate that endostatin binds to neither the latent proMMP-2 nor the HP domain but to the catalytic domain of MMP-2.

Original languageEnglish
Pages (from-to)147-152
Number of pages6
JournalFEBS Letters
Volume519
Issue number1-3
DOIs
Publication statusPublished - 2002 May 22

Fingerprint

Endostatins
Matrix Metalloproteinase 2
Catalytic Domain
Hemopexin
Matrix Metalloproteinases
Matrix Metalloproteinase Inhibitors
Surface Plasmon Resonance
Surface plasmon resonance
Hinges
Tumors
Catalyst activity
Chemical activation
progelatinase

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Lee, Seo Jin ; Jang, Jin Wook ; Kim, Young Mi ; Lee, Hyun Ick ; Jeon, Jun Yung ; Kwon, Young Guen ; Lee, Seung Taek. / Endostatin binds to the catalytic domain of matrix metalloproteinase-2. In: FEBS Letters. 2002 ; Vol. 519, No. 1-3. pp. 147-152.
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Endostatin binds to the catalytic domain of matrix metalloproteinase-2. / Lee, Seo Jin; Jang, Jin Wook; Kim, Young Mi; Lee, Hyun Ick; Jeon, Jun Yung; Kwon, Young Guen; Lee, Seung Taek.

In: FEBS Letters, Vol. 519, No. 1-3, 22.05.2002, p. 147-152.

Research output: Contribution to journalArticle

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AU - Lee, Seo Jin

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