Endothelial F-actin changes in the alkali burned rabbit cornea.

Eungkweon Kim, H. B. Kim, Y. T. Chung, I. C. Kim

Research output: Contribution to journalArticle

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Abstract

The healing mechanism of corneal endothelium after alkali burn was not completely understood. Rabbit cornea was burned with 1N sodium hydxoside for 1 minute. Endothelial F-actin was stained with NBD-phallacidin in regular sequence to find out the details of endothelial healing after alkali burn. F-actin was completely destroyed leaving a sharp margin against the unaffected area 1 hour after the burn. In the 3, 5 and 7 day specimens, highly active F-actin reactions were noted at the wound margin. New multiple F-actin layers, arising from the intact endothelium near the wound margin, were noted in the 9 day specimen. In the 8 1/2 month specimen, the endothelial defected area was covered by large primitive cells, each of which showed F-actin fiber bundles in the cytoplasm with a large nuclear shadow. Nearly all of the large primitive cells showed F-actin fibers arranged in shapes of cell junctions. Twelve months after the burn, endothelial defects were not found. Nearly all of the endothelial cells were normal in size and shape except for some mushroom-like projections toward the anterior chamber in some areas. Nineteen months after the burn, the endothelial cells were normal. Endothelial wound healing process can be continued even 1 year after the alkali burn in rabbit cornea.

Original languageEnglish
Pages (from-to)484-492
Number of pages9
JournalYonsei Medical Journal
Volume35
Issue number4
DOIs
Publication statusPublished - 1994 Jan 1

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Alkalies
Cornea
Actins
Rabbits
Endothelial Cells
Corneal Endothelium
Intercellular Junctions
Agaricales
Wounds and Injuries
Anterior Chamber
Wound Healing
Endothelium
Cytoplasm
Sodium

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Kim, Eungkweon ; Kim, H. B. ; Chung, Y. T. ; Kim, I. C. / Endothelial F-actin changes in the alkali burned rabbit cornea. In: Yonsei Medical Journal. 1994 ; Vol. 35, No. 4. pp. 484-492.
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Endothelial F-actin changes in the alkali burned rabbit cornea. / Kim, Eungkweon; Kim, H. B.; Chung, Y. T.; Kim, I. C.

In: Yonsei Medical Journal, Vol. 35, No. 4, 01.01.1994, p. 484-492.

Research output: Contribution to journalArticle

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