Engineered Attenuated Salmonella typhimurium Expressing Neoantigen Has Anticancer Effects

Jungheun Hyun, Soyeong Jun, Hyeonseob Lim, Hyunjun Cho, Sung Hwan You, Sang Jun Ha, Jung Joon Min, Duhee Bang

Research output: Contribution to journalArticlepeer-review

Abstract

Neoantigen vaccines are an immunotherapy strategy for treating cancer. The vaccine degrades quickly, so the strategy must include protection and precise targeting for immune cell stimulation. In this study, we engineered attenuated Salmonella typhimurium, which is highly infiltrative to tumors, to act as a carrier for Neoantigen peptide vaccine. Our system used a constitutive promoter vector, so that a single injection of Salmonella expressing Neoantigen could be used without requiring additional induction injections. In vivo experiments on bacteria-treated mice showed that Neoantigen expressed by the engineered carrier infiltrated tumors and resulted in suppressed tumor growth, higher survival rates and longer survival times, a relative increase of CD4 and CD8 T cells, and cytokine release. These results indicate that engineered Salmonella can be used as a carrier for Neoantigen immunotherapy.

Original languageEnglish
Pages (from-to)2478-2487
Number of pages10
JournalACS Synthetic Biology
Volume10
Issue number10
DOIs
Publication statusPublished - 2021 Oct 15

Bibliographical note

Funding Information:
This research was supported by (i) the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (NRF-2018M3A9H3024850); (ii) the Midcareer Researcher Program (NRF-2021R1A2C2094264) through the National Research Foundation of Korea (NRF), funded by the Ministry of Science, ICT and Future Planning; (iii) Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea [Grant Number: HI14C1277(HR14C0003)]; (iv) the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2019M3A9B6065221).

Publisher Copyright:
© 2021 American Chemical Society.

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Biochemistry, Genetics and Molecular Biology (miscellaneous)

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