In this study, anaerobic digestion of waste-activated sludge was bioaugmented with hydrolytic bacteria, Bacteroidetes uniformis (Bacteroidetes, B) and Clostridium sp. (Firmicutes, F) at various dosages. Bioaugmentation resulted in enhanced methane conversion of waste-activated sludge. The highest methane yield of 298.1 mL CH4/g-COD, 85.2% COD conversion efficiency was obtained when Bacteroidetes uniformis and Clostridium sp. were augmented at 100 and 900 CFU/mL, respectively. The microbial community analysis demonstrated that bioaugmentation increased the proportion of Bacteroidetes, Firmicutes, and Proteobacteria. Furthermore, at the highest methane yield, the principal methanogenic pathway was altered from acetoclastic to a mixture of hydrogenotrophic and acetoclastic; the major species shifted from Methanosaeta concilii to Methanobacterium subterraneum. Predicted gene analysis revealed that increased expression of hydrolases resulted in enhanced methane conversion through bioaugmentation.
|Publication status||Published - 2021 Jul|
Bibliographical noteFunding Information:
This work was supported by Korea Environment Industry & Technology Institute (KEITI) through Project for developing innovative drinking water and wastewater technologies, funded by Korea Ministry of Environment (MOE) ( ARQ202001174001 ).
© 2021 Elsevier Ltd
All Science Journal Classification (ASJC) codes
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
- Waste Management and Disposal