Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]

Xanthi Tsampoula, Michael Mazilu, Tom Vettenburg, Frank Gunn-Moore, Kishan Dholakia

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.

Original languageEnglish
Pages (from-to)42-46
Number of pages5
JournalPhotonics Research
Volume1
Issue number1
DOIs
Publication statusPublished - 2013 Jan 1

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Mitochondria
Plasmids
Diffraction
Cells
ovaries
plasmids
mitochondria
hamsters
cells
diffraction
fluorescent protein 583

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Tsampoula, Xanthi ; Mazilu, Michael ; Vettenburg, Tom ; Gunn-Moore, Frank ; Dholakia, Kishan. / Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]. In: Photonics Research. 2013 ; Vol. 1, No. 1. pp. 42-46.
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Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]. / Tsampoula, Xanthi; Mazilu, Michael; Vettenburg, Tom; Gunn-Moore, Frank; Dholakia, Kishan.

In: Photonics Research, Vol. 1, No. 1, 01.01.2013, p. 42-46.

Research output: Contribution to journalArticle

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