Abstract
We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.
| Original language | English |
|---|---|
| Pages (from-to) | 42-46 |
| Number of pages | 5 |
| Journal | Photonics Research |
| Volume | 1 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 Jan 1 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
Cite this
}
Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]. / Tsampoula, Xanthi; Mazilu, Michael; Vettenburg, Tom; Gunn-Moore, Frank; Dholakia, Kishan.
In: Photonics Research, Vol. 1, No. 1, 01.01.2013, p. 42-46.Research output: Contribution to journal › Article
TY - JOUR
T1 - Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]
AU - Tsampoula, Xanthi
AU - Mazilu, Michael
AU - Vettenburg, Tom
AU - Gunn-Moore, Frank
AU - Dholakia, Kishan
PY - 2013/1/1
Y1 - 2013/1/1
N2 - We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.
AB - We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.
UR - http://www.scopus.com/inward/record.url?scp=84891359403&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891359403&partnerID=8YFLogxK
U2 - 10.1364/PRJ.1.000042
DO - 10.1364/PRJ.1.000042
M3 - Article
AN - SCOPUS:84891359403
VL - 1
SP - 42
EP - 46
JO - Photonics Research
JF - Photonics Research
SN - 2327-9125
IS - 1
ER -