Abstract
We show that superoscillating light fields, created using the method of optical eigenmodes, enable more efficient multiphoton-mediated cell transfection. Chinese hamster ovary cells are transfected with a plasmid and exhibit expression of DsRed-Mito in the mitochondria. We demonstrate an efficiency improvement of 35% compared to the diffraction-limited spot. This opens up new vistas for nanoscale localized cell transfection.
| Original language | English |
|---|---|
| Pages (from-to) | 42-46 |
| Number of pages | 5 |
| Journal | Photonics Research |
| Volume | 1 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2013 Jan 1 |
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
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Tsampoula, X., Mazilu, M., Vettenburg, T., Gunn-Moore, F., & Dholakia, K. (2013). Enhanced cell transfection using subwavelength focused optical eigenmode beams [Invited]. Photonics Research, 1(1), 42-46. https://doi.org/10.1364/PRJ.1.000042