Abstract
Poly-γ-glutamic acid (γ-PGA), which is produced by microbial fermentation, is a biodegradable, hydrophilic and non-toxic biomaterial. γ-PGA has many carboxyl groups that makes it a polyanionic biopolymer with swelling ability, biocompatibility and anticoagulant activity. On the other hand, few studies have examined the effect of γ-PGA on the cellular activity of human umbilical vein endothelial cells (HUVECs). The present study evaluated the effects of γ-PGA and fabricated γ-PGA/PU composite films on the attachment and proliferation of HUVECs after treatment with microwave plasma at atmospheric pressure. The results confirmed that γ-PGA is capable of increasing the proliferation and differentiation of HUVECs to form capillary tubes with enhanced alignment and organization. Moreover, the microwave plasma modified γ-PGA/PU composite film was more hydrophilic and the surface roughness was enhanced. In addition, the attachment and proliferation of the HUVECs were increased by the plasma treatment. These results suggest that γ-PGA and surface modified γ-PGA/PU composite can be applied as bioactive and biocompatible materials in vascular tissue engineering but further testing of γ-PGA and γ-PGA/PU composite films will be needed to confirm the potential effects for use in vascular applications. [InlineMediaObject not available: see fulltext.]
| Original language | English |
|---|---|
| Pages (from-to) | 537-541 |
| Number of pages | 5 |
| Journal | Macromolecular Research |
| Volume | 19 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2011 Jun 1 |
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All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)
- Organic Chemistry
- Polymers and Plastics
- Materials Chemistry
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Enhanced cellular responses of vascular endothelial cells on poly-γ-glutamic acid/PU composite film treated with microwave-induced plasma at atmospheric pressure. / Park, Bong Joo; Kwon, Byoung Ju; Kang, Jae Kyeong; Lee, Mi Hee; Han, Inho; Kim, Jeong Koo; Park, Jong Chul.
In: Macromolecular Research, Vol. 19, No. 6, 01.06.2011, p. 537-541.Research output: Contribution to journal › Article
TY - JOUR
T1 - Enhanced cellular responses of vascular endothelial cells on poly-γ-glutamic acid/PU composite film treated with microwave-induced plasma at atmospheric pressure
AU - Park, Bong Joo
AU - Kwon, Byoung Ju
AU - Kang, Jae Kyeong
AU - Lee, Mi Hee
AU - Han, Inho
AU - Kim, Jeong Koo
AU - Park, Jong Chul
PY - 2011/6/1
Y1 - 2011/6/1
N2 - Poly-γ-glutamic acid (γ-PGA), which is produced by microbial fermentation, is a biodegradable, hydrophilic and non-toxic biomaterial. γ-PGA has many carboxyl groups that makes it a polyanionic biopolymer with swelling ability, biocompatibility and anticoagulant activity. On the other hand, few studies have examined the effect of γ-PGA on the cellular activity of human umbilical vein endothelial cells (HUVECs). The present study evaluated the effects of γ-PGA and fabricated γ-PGA/PU composite films on the attachment and proliferation of HUVECs after treatment with microwave plasma at atmospheric pressure. The results confirmed that γ-PGA is capable of increasing the proliferation and differentiation of HUVECs to form capillary tubes with enhanced alignment and organization. Moreover, the microwave plasma modified γ-PGA/PU composite film was more hydrophilic and the surface roughness was enhanced. In addition, the attachment and proliferation of the HUVECs were increased by the plasma treatment. These results suggest that γ-PGA and surface modified γ-PGA/PU composite can be applied as bioactive and biocompatible materials in vascular tissue engineering but further testing of γ-PGA and γ-PGA/PU composite films will be needed to confirm the potential effects for use in vascular applications. [InlineMediaObject not available: see fulltext.]
AB - Poly-γ-glutamic acid (γ-PGA), which is produced by microbial fermentation, is a biodegradable, hydrophilic and non-toxic biomaterial. γ-PGA has many carboxyl groups that makes it a polyanionic biopolymer with swelling ability, biocompatibility and anticoagulant activity. On the other hand, few studies have examined the effect of γ-PGA on the cellular activity of human umbilical vein endothelial cells (HUVECs). The present study evaluated the effects of γ-PGA and fabricated γ-PGA/PU composite films on the attachment and proliferation of HUVECs after treatment with microwave plasma at atmospheric pressure. The results confirmed that γ-PGA is capable of increasing the proliferation and differentiation of HUVECs to form capillary tubes with enhanced alignment and organization. Moreover, the microwave plasma modified γ-PGA/PU composite film was more hydrophilic and the surface roughness was enhanced. In addition, the attachment and proliferation of the HUVECs were increased by the plasma treatment. These results suggest that γ-PGA and surface modified γ-PGA/PU composite can be applied as bioactive and biocompatible materials in vascular tissue engineering but further testing of γ-PGA and γ-PGA/PU composite films will be needed to confirm the potential effects for use in vascular applications. [InlineMediaObject not available: see fulltext.]
UR - http://www.scopus.com/inward/record.url?scp=79960065510&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79960065510&partnerID=8YFLogxK
U2 - 10.1007/s13233-011-0605-6
DO - 10.1007/s13233-011-0605-6
M3 - Article
AN - SCOPUS:79960065510
VL - 19
SP - 537
EP - 541
JO - Macromolecular Research
JF - Macromolecular Research
SN - 1598-5032
IS - 6
ER -