Enhanced cellular transfection by ternary non-viral gene vectors coupled with adeno-associated virus-derived peptides

Ji Seon Oh, Minah Park, Jung Suk Kim, Jae-Hyung Jang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The establishment of efficient and safe gene delivery systems is crucial for biomedical applications. To address this objective, novel, ternary hybrid gene vectors are designed with viral capsid peptides in non-viral gene carriers. The viral peptide, TQVGQKT, is coupled with a membrane active peptide, LK15. Which acts as a linker to tag peptide with plasmid DNA. Additionally, polyethylenimine (PEI) is employed to condense the complexes further, thereby forming ternary DNA/TQVGQKT-LK15/PEI complexes. The ternary complexes result in rapid internalization leading to significantly enhanced cellular transfection. The new moiety, TQVGQKT, as well as enhanced cellular transfection, will certainly provide crucial insights for the design of novel non-viral gene carriers with efficient and safe properties.

Original languageEnglish
Pages (from-to)121-130
Number of pages10
JournalMacromolecular Bioscience
Volume14
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Dependovirus
Viruses
Peptides
Transfection
Genes
Polyethyleneimine
DNA
Gene Transfer Techniques
Capsid
Plasmids
Membranes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

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abstract = "The establishment of efficient and safe gene delivery systems is crucial for biomedical applications. To address this objective, novel, ternary hybrid gene vectors are designed with viral capsid peptides in non-viral gene carriers. The viral peptide, TQVGQKT, is coupled with a membrane active peptide, LK15. Which acts as a linker to tag peptide with plasmid DNA. Additionally, polyethylenimine (PEI) is employed to condense the complexes further, thereby forming ternary DNA/TQVGQKT-LK15/PEI complexes. The ternary complexes result in rapid internalization leading to significantly enhanced cellular transfection. The new moiety, TQVGQKT, as well as enhanced cellular transfection, will certainly provide crucial insights for the design of novel non-viral gene carriers with efficient and safe properties.",
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Enhanced cellular transfection by ternary non-viral gene vectors coupled with adeno-associated virus-derived peptides. / Oh, Ji Seon; Park, Minah; Kim, Jung Suk; Jang, Jae-Hyung.

In: Macromolecular Bioscience, Vol. 14, No. 1, 01.01.2014, p. 121-130.

Research output: Contribution to journalArticle

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