Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma

Young Woo Cheon, Won Jai Lee, Hyun Sook Baek, Young Dae Lee, Jongchul Park, Young Hwan Park, Chang Seok Ki, Kie Hyung Chung, Dong Kyun Rah

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Silk fibroin (SF) is a natural, degradable, fibrous protein that is biocompatible, is easily processed, and possesses unique mechanical properties. Another natural material, wool keratose (WK), is a soluble derivative of wool keratin, containing amino acid sequences that induce cell adhesion. Here, we blended SF and WK to improve the poor electrospinability of WK and increase the adhesiveness of SF. We hypothesized that microwave-induced argon plasma treatment would improve chondrogenic cell growth and cartilage-specific extracellular matrix formation on a three-dimensional SF/WK scaffold. After argon plasma treatment, static water contact angle measurement revealed increased hydrophilicity of the SF/WK scaffold, and scanning electron microscopy showed that treated SF/WK scaffolds had deeper and more cylindrical pores than nontreated scaffolds. Attachment and proliferation of neonatal human knee articular chondrocytes on treated SF/WK scaffolds increased significantly, followed by increased glycosaminoglycan synthesis. Our results suggest that microwave-induced, plasma-treated SF/WK scaffolds have potential in cartilage tissue engineering.

Original languageEnglish
Pages (from-to)384-392
Number of pages9
JournalArtificial Organs
Volume34
Issue number5
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

Fibroins
Silk
Wool
Argon
Chondrocytes
Microwaves
Scaffolds
Joints
Plasmas
Cartilage
Scleroproteins
Keratin
Adhesiveness
Cell adhesion
Bioelectric potentials
Hydrophilicity
Cell growth
Tissue Engineering
Scaffolds (biology)
Angle measurement

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Cheon, Young Woo ; Lee, Won Jai ; Baek, Hyun Sook ; Lee, Young Dae ; Park, Jongchul ; Park, Young Hwan ; Ki, Chang Seok ; Chung, Kie Hyung ; Rah, Dong Kyun. / Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma. In: Artificial Organs. 2010 ; Vol. 34, No. 5. pp. 384-392.
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Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma. / Cheon, Young Woo; Lee, Won Jai; Baek, Hyun Sook; Lee, Young Dae; Park, Jongchul; Park, Young Hwan; Ki, Chang Seok; Chung, Kie Hyung; Rah, Dong Kyun.

In: Artificial Organs, Vol. 34, No. 5, 01.01.2010, p. 384-392.

Research output: Contribution to journalArticle

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