Enhanced cross protection by hetero prime-boost vaccination with recombinant influenza viruses containing chimeric hemagglutinin-M2e epitopes

Bo Ryoung Park, Jeeva Subbiah, Ki Hye Kim, Young Man Kwon, Judy Oh, Min Chul Kim, Chong Hyun Shin, Baik Lin Seong, Sang Moo Kang

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Annual repeat influenza vaccination raises concerns about protective efficacy against mismatched viruses. We investigated the impact of heterologous prime-boost vaccination on inducing cross protection by designing recombinant influenza viruses with chimeric hemagglutinin (HA) carrying M2 extracellular domains (M2e-HA). Heterologous prime-boost vaccination of C57BL/6 mice with M2e-HA chimeric virus more effectively induced M2e and HA stalk specific IgG antibodies correlating with cross protection than homologous prime-boost vaccination. Induction of M2e and HA stalk specific IgG antibodies was compromised in 1-year old mice, indicating significant aging effects on priming subdominant M2e and HA stalk IgG antibody responses. This study demonstrates that a heterologous prime-boost strategy with recombinant influenza virus expressing extra M2e epitopes provides more effective cross protection than homologous vaccination.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalVirology
Volume566
DOIs
Publication statusPublished - 2022 Jan

Bibliographical note

Funding Information:
This study was supported by NIH/NIAID grants AI093772 (S.M.K.), AI154656 (S.M.K.), and AI147042 (S.M.K). The following reagent was obtained from International Reagent Resource /CDC: Reassortant A/Switzerland/9715293/2013 virus ( X-247 rgH3N2 , FR-1366 ) which carries the A/PR8 backbone genes, and H3 HA and N2 NA genes from A/Switzerland/9715293/2013.

Funding Information:
This study was supported by NIH/NIAID grants AI093772 (S.M.K.), AI154656 (S.M.K.), and AI147042 (S.M.K). The following reagent was obtained from International Reagent Resource/CDC: Reassortant A/Switzerland/9715293/2013 virus (X-247 rgH3N2, FR-1366) which carries the A/PR8 backbone genes, and H3 HA and N2 NA genes from A/Switzerland/9715293/2013.

Publisher Copyright:
© 2021 Elsevier Inc.

All Science Journal Classification (ASJC) codes

  • Virology

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