Enhanced image reconstruction of three-dimensional fluorescent assays by subtractive structured-light illumination microscopy

Jong Ryul Choi, Donghyun Kim

Research output: Contribution to journalArticle

10 Citations (Scopus)


We investigate improved image reconstruction of structured light illumination for high-resolution imaging of three-dimensional (3D) cell-based assays. For proof of concept, an in situ fluorescence optical detection system was built with a digital micromirror device as a spatial light modulator, for which phase and tilting angle in a grid pattern were varied to implement specific image reconstruction schemes. Subtractive reconstruction algorithms based on structured light illumination were used to acquire images of fluorescent microbeads deposited as a two-dimensional monolayer or in 3D alginate matrix. We have confirmed that an optical subtraction algorithm improves axial and lateral resolution by effectively removing out-of-focus fluorescence. The results suggest that subtractive image reconstruction can be useful for structured illumination microscopy of broad types of cell-based assays with high image resolution.

Original languageEnglish
Pages (from-to)2165-2173
Number of pages9
JournalJournal of the Optical Society of America A: Optics and Image Science, and Vision
Issue number10
Publication statusPublished - 2012 Oct 1


All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Computer Vision and Pattern Recognition

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