Enhanced lateral resolution in continuous wave stimulated emission depletion microscopy using tightly focused annular radially polarized excitation beam

Geon Lim, Wan Chin Kim, Seunghee Oh, Hyungsuk Lee, No Cheol Park

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The lateral resolution of continuous wave (CW) stimulated emission depletion (STED) microscopy is enhanced about 12% by applying annular-shaped amplitude modulation to the radially polarized excitation beam. A focused annularly filtered radially polarized excitation beam provides a more condensed point spread function (PSF), which contributes to enhance effective STED resolution of CW STED microscopy. Theoretical analysis shows that the FWHM of the effective PSF on the detection plane is smaller than for conventional CW STED. Simulation shows the donut-shaped PSF of the depletion beam and confocal optics suppress undesired PSF sidelobes. Imaging experiments agree with the simulated resolution improvement.

Original languageEnglish
Article numbere201900060
JournalJournal of Biophotonics
Volume12
Issue number9
DOIs
Publication statusPublished - 2019 Sep 1

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Stimulated emission
Optical transfer function
stimulated emission
continuous radiation
Microscopy
point spread functions
Microscopic examination
depletion
microscopy
excitation
Amplitude modulation
Full width at half maximum
sidelobes
Optics
Imaging techniques
optics
Experiments
simulation

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

Cite this

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title = "Enhanced lateral resolution in continuous wave stimulated emission depletion microscopy using tightly focused annular radially polarized excitation beam",
abstract = "The lateral resolution of continuous wave (CW) stimulated emission depletion (STED) microscopy is enhanced about 12{\%} by applying annular-shaped amplitude modulation to the radially polarized excitation beam. A focused annularly filtered radially polarized excitation beam provides a more condensed point spread function (PSF), which contributes to enhance effective STED resolution of CW STED microscopy. Theoretical analysis shows that the FWHM of the effective PSF on the detection plane is smaller than for conventional CW STED. Simulation shows the donut-shaped PSF of the depletion beam and confocal optics suppress undesired PSF sidelobes. Imaging experiments agree with the simulated resolution improvement.",
author = "Geon Lim and Kim, {Wan Chin} and Seunghee Oh and Hyungsuk Lee and Park, {No Cheol}",
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language = "English",
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Enhanced lateral resolution in continuous wave stimulated emission depletion microscopy using tightly focused annular radially polarized excitation beam. / Lim, Geon; Kim, Wan Chin; Oh, Seunghee; Lee, Hyungsuk; Park, No Cheol.

In: Journal of Biophotonics, Vol. 12, No. 9, e201900060, 01.09.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Enhanced lateral resolution in continuous wave stimulated emission depletion microscopy using tightly focused annular radially polarized excitation beam

AU - Lim, Geon

AU - Kim, Wan Chin

AU - Oh, Seunghee

AU - Lee, Hyungsuk

AU - Park, No Cheol

PY - 2019/9/1

Y1 - 2019/9/1

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AB - The lateral resolution of continuous wave (CW) stimulated emission depletion (STED) microscopy is enhanced about 12% by applying annular-shaped amplitude modulation to the radially polarized excitation beam. A focused annularly filtered radially polarized excitation beam provides a more condensed point spread function (PSF), which contributes to enhance effective STED resolution of CW STED microscopy. Theoretical analysis shows that the FWHM of the effective PSF on the detection plane is smaller than for conventional CW STED. Simulation shows the donut-shaped PSF of the depletion beam and confocal optics suppress undesired PSF sidelobes. Imaging experiments agree with the simulated resolution improvement.

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