TY - JOUR
T1 - Enhanced operation of femtosecond lasers and applications in cell transfection
AU - Brown, Christian T.A.
AU - Stevenson, David J.
AU - Tsampoula, Xanthi
AU - McDougall, Craig
AU - Lagatsky, Alexander A.
AU - Sibbett, Wilson
AU - Gunn-Moore, Frank J.
AU - Dholakia, Kishan
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2008
Y1 - 2008
N2 - In this work we present a review and discussion on the enhancement of femtosecond (fs) lasers for use within biophotonics with a particular focus on their use in optical transfection techniques. We describe the broad range of source options now available for the generation of femtosecond pulses before briefly reviewing the application of fs laser in optical transfection studies. We show that major performance enhancements may be obtained by optimising the spatial and temporal performance of the laser source before considering possible future directions in this field. In relation to optical transfection we describe how such laser sources initiate a multiphoton process to permeate the cell membrane in a transient fashion. We look at aspects of this technique including the ability to combine transfection with optical trapping. For future implementation of such transfection we explore the role of new sources and "nondiffracting" light fields.
AB - In this work we present a review and discussion on the enhancement of femtosecond (fs) lasers for use within biophotonics with a particular focus on their use in optical transfection techniques. We describe the broad range of source options now available for the generation of femtosecond pulses before briefly reviewing the application of fs laser in optical transfection studies. We show that major performance enhancements may be obtained by optimising the spatial and temporal performance of the laser source before considering possible future directions in this field. In relation to optical transfection we describe how such laser sources initiate a multiphoton process to permeate the cell membrane in a transient fashion. We look at aspects of this technique including the ability to combine transfection with optical trapping. For future implementation of such transfection we explore the role of new sources and "nondiffracting" light fields.
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U2 - 10.1002/jbio.200810011
DO - 10.1002/jbio.200810011
M3 - Article
C2 - 19412968
AN - SCOPUS:51149106468
VL - 1
SP - 183
EP - 199
JO - Journal of Biophotonics
JF - Journal of Biophotonics
SN - 1864-063X
IS - 3
ER -