TY - JOUR
T1 - Enhanced Optical Manipulation of Cells Using Antireflection Coated Microparticles
AU - Craig, Derek
AU - McDonald, Alison
AU - Mazilu, Michael
AU - Rendall, Helen
AU - Gunn-Moore, Frank
AU - Dholakia, Kishan
N1 - Publisher Copyright:
© 2015 American Chemical Society.
Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 2015/10/21
Y1 - 2015/10/21
N2 - We demonstrate the use of antireflection (AR) coated microparticles for the enhanced optical manipulation of cells. Specifically, we incubate CHO-K1, HL60, and NMuMG cell lines with AR-coated titania microparticles and subsequently performed drag force measurements using optical trapping. Direct comparisons were performed between native, polystyrene microparticle and AR microparticle tagged cells. The optical trapping efficiency was recorded by measuring the Q value in a drag force experiment. CHO-K1 cells incubated with AR microparticles show an increase in the Q value of nearly 220% versus native cells. With the inclusion of AR microparticles, cell velocities exceeding 50 μm/s were recorded for only 33 mW of laser trapping power. Cell viability was confirmed with fluorescent dyes and cells expressing a fluorescent ubiquitination-based cell cycle protein (FUCCI), which verified no disruption to the cell cycle in the presence of AR microparticles.
AB - We demonstrate the use of antireflection (AR) coated microparticles for the enhanced optical manipulation of cells. Specifically, we incubate CHO-K1, HL60, and NMuMG cell lines with AR-coated titania microparticles and subsequently performed drag force measurements using optical trapping. Direct comparisons were performed between native, polystyrene microparticle and AR microparticle tagged cells. The optical trapping efficiency was recorded by measuring the Q value in a drag force experiment. CHO-K1 cells incubated with AR microparticles show an increase in the Q value of nearly 220% versus native cells. With the inclusion of AR microparticles, cell velocities exceeding 50 μm/s were recorded for only 33 mW of laser trapping power. Cell viability was confirmed with fluorescent dyes and cells expressing a fluorescent ubiquitination-based cell cycle protein (FUCCI), which verified no disruption to the cell cycle in the presence of AR microparticles.
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U2 - 10.1021/acsphotonics.5b00178
DO - 10.1021/acsphotonics.5b00178
M3 - Article
AN - SCOPUS:84945567686
VL - 2
SP - 1403
EP - 1409
JO - ACS Photonics
JF - ACS Photonics
SN - 2330-4022
IS - 10
ER -