Enhanced production of manganese peroxidase from immobilized Phanerochaete chrysosporium due to the increased autolysis of chlamydospore-like cells

Eunyong Chung, Junse Oh, Sangpill Hwang, Ik Sung Ahn, Joon Yoon Yeo

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The autolysis of chlamydospore-like cells in Phanerochaete chrysosporium immobilized in polyurethane foam correlated with the production of manganese peroxidase (MnP). The maximum specific activity of MnP was 1055 U g dry mycelium-1 in the immobilized culture, compared with 260 U g dry mycelium-1 in the submerged culture. Scattered mycelial pellets were formed in the immobilized culture in which almost all of the chlamydospore-like cells were subject to autolysis. However, highly crowded pellets were formed in the free culture, in which only the chlamydospore-like cells in the exterior were subject to autolysis. We propose that the enhanced production of MnP in immobilized cultures of P. chrysosporium is due to increased autolysis of the chlamydospore-like cells.

Original languageEnglish
Pages (from-to)477-481
Number of pages5
JournalBiotechnology Letters
Volume27
Issue number7
DOIs
Publication statusPublished - 2005 Apr 1

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manganese peroxidase
Phanerochaete
Autolysis
Manganese
Mycelium
Polyurethanes
Foams

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

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abstract = "The autolysis of chlamydospore-like cells in Phanerochaete chrysosporium immobilized in polyurethane foam correlated with the production of manganese peroxidase (MnP). The maximum specific activity of MnP was 1055 U g dry mycelium-1 in the immobilized culture, compared with 260 U g dry mycelium-1 in the submerged culture. Scattered mycelial pellets were formed in the immobilized culture in which almost all of the chlamydospore-like cells were subject to autolysis. However, highly crowded pellets were formed in the free culture, in which only the chlamydospore-like cells in the exterior were subject to autolysis. We propose that the enhanced production of MnP in immobilized cultures of P. chrysosporium is due to increased autolysis of the chlamydospore-like cells.",
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Enhanced production of manganese peroxidase from immobilized Phanerochaete chrysosporium due to the increased autolysis of chlamydospore-like cells. / Chung, Eunyong; Oh, Junse; Hwang, Sangpill; Ahn, Ik Sung; Yeo, Joon Yoon.

In: Biotechnology Letters, Vol. 27, No. 7, 01.04.2005, p. 477-481.

Research output: Contribution to journalArticle

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T1 - Enhanced production of manganese peroxidase from immobilized Phanerochaete chrysosporium due to the increased autolysis of chlamydospore-like cells

AU - Chung, Eunyong

AU - Oh, Junse

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AU - Ahn, Ik Sung

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