Enhanced tumor cell separation by surfaces functionalized with combinations of bioadhesive proteins

Ja Hye Myung, Cari A. Launiere, Khyati A. Gajjar, David T. Eddington, Seungpyo Hong

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have achieved the evenly distributed, stable immobilization of proteins: P-selectin, E-selectin, anti-EpCAM, and mixtures of the proteins, using epoxy-functionalized glass slides. The immobilized proteins maintained their own biological adhesive functions that induce cell rolling and stationary binding. The enhanced separation capacity and capture efficiency by combination of these immobilized proteins hold promise to be potentially utilized for future cell specific capturing devices. We are presently translating these results to a device to capture CTCs from the mixture of other cell lines and whole blood. In addition to the potential use of this device as a metastatic cancer treatment tool by filtering CTCs from the bloodstream, the advantages of this device include the ability to collect CTCs from whole blood under continuous flow without labeling or damaging the CTCs. Therefore, the collected CTCs can be extracted and potentially be subject of further analysis such as genetic understanding and responses for currently available therapeutic drugs by culture expansion.

Original languageEnglish
Title of host publicationProceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010
Pages125-126
Number of pages2
Publication statusPublished - 2010 Aug 4
Event1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010 - Houston, TX, United States
Duration: 2010 Feb 72010 Feb 10

Other

Other1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010
CountryUnited States
CityHouston, TX
Period10/2/710/2/10

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Medicine(all)

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  • Cite this

    Myung, J. H., Launiere, C. A., Gajjar, K. A., Eddington, D. T., & Hong, S. (2010). Enhanced tumor cell separation by surfaces functionalized with combinations of bioadhesive proteins. In Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010 (pp. 125-126)