Enhancement of angiogenic efficacy of human cord blood cell transplantation

Seung-Woo Cho, So Jung Gwak, Sun Woong Kang, Suk Ho Bhang, Kang Won Song, Yoon Sun Yang, Cha Yong Choi, Byung Soo Kim

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We tested the hypotheses that angiogenic efficacy of cord blood mononuclear cell (CBMNC) transplantation would be enhanced by using matrix and that combined therapy of CBMNC transplantation using matrix and sustained delivery of basic fibroblast growth factor (bFGF) would be synergistic in angiogenesis induction in ischemic limbs. One day after surgical induction of hindlimb ischemia, C57BL/6J mice were randomized to receive either medium injection, CBMNC transplantation using medium, CBMNC transplantation using fibrin matrix, sustained delivery of bFGF, or a combination of sustained delivery of bFGF and CBMNC transplantation using fibrin matrix. Four weeks after treatment, the angiogenic efficacy of the treatments was evaluated by immunohistochemical examinations and microvessel density determination in the ischemic sites. Transplanted CBMNCs survived, proliferated, and participated in capillary formation in ischemic limbs. CBMNC transplantation using fibrin matrix significantly increased the densities of capillaries and arterioles compared with CBMNC transplantation using medium. Importantly, combined therapy of sustained delivery of bFGF and CBMNC transplantation using fibrin matrix further increased the densities of capillaries and arterioles compared with either therapy alone. The angiogenic efficacy of angiogenic cell transplantation is enhanced by cell transplantation using matrix. Combined therapy of sustained release of angiogenic protein and angiogenic cell transplantation synergistically enhances angiogenesis in ischemic limbs compared to each therapy separately.

Original languageEnglish
Pages (from-to)1651-1661
Number of pages11
JournalTissue Engineering
Volume12
Issue number6
DOIs
Publication statusPublished - 2006 Jun 1

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Cell Transplantation
Fetal Blood
Blood Cells
Blood
Cells
Fibroblasts
Fibroblast Growth Factor 2
Fibrin
Extremities
Arterioles
Therapeutics
Angiogenic Proteins
Hindlimb
Microvessels
Inbred C57BL Mouse
Proteins
Intercellular Signaling Peptides and Proteins
Ischemia

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Cell Biology

Cite this

Cho, S-W., Gwak, S. J., Kang, S. W., Bhang, S. H., Song, K. W., Yang, Y. S., ... Kim, B. S. (2006). Enhancement of angiogenic efficacy of human cord blood cell transplantation. Tissue Engineering, 12(6), 1651-1661. https://doi.org/10.1089/ten.2006.12.1651
Cho, Seung-Woo ; Gwak, So Jung ; Kang, Sun Woong ; Bhang, Suk Ho ; Song, Kang Won ; Yang, Yoon Sun ; Choi, Cha Yong ; Kim, Byung Soo. / Enhancement of angiogenic efficacy of human cord blood cell transplantation. In: Tissue Engineering. 2006 ; Vol. 12, No. 6. pp. 1651-1661.
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Cho, S-W, Gwak, SJ, Kang, SW, Bhang, SH, Song, KW, Yang, YS, Choi, CY & Kim, BS 2006, 'Enhancement of angiogenic efficacy of human cord blood cell transplantation', Tissue Engineering, vol. 12, no. 6, pp. 1651-1661. https://doi.org/10.1089/ten.2006.12.1651

Enhancement of angiogenic efficacy of human cord blood cell transplantation. / Cho, Seung-Woo; Gwak, So Jung; Kang, Sun Woong; Bhang, Suk Ho; Song, Kang Won; Yang, Yoon Sun; Choi, Cha Yong; Kim, Byung Soo.

In: Tissue Engineering, Vol. 12, No. 6, 01.06.2006, p. 1651-1661.

Research output: Contribution to journalArticle

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