Enhancement of image quality and imaging depth with airy light-sheet microscopy in cleared and non-cleared neural tissue

Jonathan Nylk, Kaley McCluskey, Sanya Aggarwal, Javier A. Tello, Kishan Dholakia

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.

Original languageEnglish
Article number#270350
Pages (from-to)4021-4033
Number of pages13
JournalBiomedical Optics Express
Volume7
Issue number10
DOIs
Publication statusPublished - 2016 Oct 1

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Image Enhancement
Microscopy
microscopy
Light
augmentation
penetration
clearing
Fourier analysis
Fourier Analysis
Lighting
aberration
illumination
probes
scattering

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Nylk, Jonathan ; McCluskey, Kaley ; Aggarwal, Sanya ; Tello, Javier A. ; Dholakia, Kishan. / Enhancement of image quality and imaging depth with airy light-sheet microscopy in cleared and non-cleared neural tissue. In: Biomedical Optics Express. 2016 ; Vol. 7, No. 10. pp. 4021-4033.
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Enhancement of image quality and imaging depth with airy light-sheet microscopy in cleared and non-cleared neural tissue. / Nylk, Jonathan; McCluskey, Kaley; Aggarwal, Sanya; Tello, Javier A.; Dholakia, Kishan.

In: Biomedical Optics Express, Vol. 7, No. 10, #270350, 01.10.2016, p. 4021-4033.

Research output: Contribution to journalArticle

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